Fundamentals of Fluorescence Microscopy

Alberto Diaspro, born in Genoa, Italy, on April 7, 1959, received his Laurea in Electronic Engineering in 1983, Univ.of Genoa. Dir. Nanophys at Italian Institute of Technology (IIT), Deputy Director of IIT, Prof. Appl. Phys. at Univ.of Genoa. President of Optics Within Life Sciences, IEEE Senior member and Editor in chief of Microscopy Research and Technique (7/2013). Alberto Diaspro is a member of the Bioengineering and  Robotics  PhD Advisory Committee (University of Genova) and  affiliated with the Institute of Biophysics of the National Research Council (CNR). He founded LAMBS in 2003 (Laboratory for Advanced Microscopy, Bioimaging and Spectroscopy, www.lambs.it). President of EBSA (European Biophysical Societies' Association, www.ebsa.org) (2009?2011), President of OWLS (Optics with Life Sciences) (2010?2014) and member of the International Relations Committee of the Biophysical Society. ISI Pubs > 250, H=30, cit > 4000 (Google Scholar). AD isinvolved in research development of instrumentation for applications in biophysics and biomedical engineering and in nanobiotechnological projects. Partha Pratim Mondal is an assistant professor at the Indian Institute of Science in the department of Instrumentation and Applied Physics. He received his Ph.D. in Physics from the Indian Institute of Science, Bangalore, India in 2005. He has been a researcher at the MIT, Cambridge, MA, USA and at the universities of Genova, Italy and also was a ICTP Fellow, Trieste, Italy. He has more than 30 journal publications to his name and 18 contributions to conference proceedings. Currently, he is Associate Editor for Scientific Reports (Nature Publishing) , Academic Editor to the AIP Advances journal and an editorial board member to ‘Microscopy, Research and Technique (Wiley).

Preface.- Acknowledgements.- Dedication.- Ray Optics, Wave Optics and Imaging System Designing.- Basics of Electromagnetic Theory for Fluorescence Microscopy.- Electric Field Effects in Optical Microscopy Systems.- Quantum Description of Radiation Field and Optical Microscopy.- Molecular Physics of Fluorescence Markers.- Basics of Fluorescence and Photophysics.- General Fluorescence Imaging Techniques.- Multiphoton Fluorescence Microscopy.- Super Resolution Fluorescence Microscopy.- Image Reconstruction Methodologies for Fluorescence Microscopy.-  Future Prospective of Fluorescence Microscopy.- Appendix I.- Appendix II.- Appendix III.