Plant Secondary Metabolism Engineering

Plant Secondary Metabolism and Challenges in Modifying Its Operation: An Overview.- Suppression Subtractive Hybridization as a Tool to Identify Anthocyanin Metabolism-Related Genes in Apple Skin.- Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System.- Site-Directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis.- Isolation of Proteins Binding to Promoter Elements of Alkaloid Metabolism-Related Genes Using Yeast One-Hybrid.- Modulation of Carotenoid Accumulation in Transgenic Potato by Inducing Chromoplast Formation with Enhanced Sink Strength.- Over-expression of Rate-Limiting Enzymes to Improve Alkaloid Productivity.- Microbial Expression of Alkaloid Biosynthetic Enzymes for Characterization of Their Properties.- Producing a Recombinant Flavin-Containing Monooxygenase from Coffea arabica in Escherichia coli for Screening of Potential Natural Substrates.- Efficient Production of Active Form Recombinant Cassava Hydroxynitrile Lyase Using Escherichia coli in Low-Temperature Culture.- of the Early Pathway to Taxol Biosynthesis in Yeast by Means of Biosynthetic Gene Cluster Construction Using SOE-PCR and Homologous Recombination.- Biocatalytic Synthesis of Tritium (3H)-Labelled Taxa-4(5),11(12)-diene, the Pathway Committing Precursor of the Taxoid Diterpenoids.- USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories.- Enrichment of Carotenoids in Flaxseed by Introducing a Bacterial Phytoene Synthase Gene.- Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants.- Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures Through Transformation with Rol Genes.- Flow Cytometric Methods to Investigate Culture Heterogeneities for Plant Metabolic Engineering.- Phenylpropanoid Biosynthesis in Leaves and Glandular Trichomes of Basil (Ocimum basilicum L.).- Fusion with Fluorescent Proteins for Subcellular Localization of Enzymes Involved in Plant Alkaloid Biosynthesis.- Immunohistochemical Localisation of a Putative Flavonoid Transporter in Grape Berries.- Electrogenic Bromosulfalein Transport in Isolated Membrane Vesicles: Implementation in Both Animal and Plant Preparations for the Study of Flavonoid Transporters.