Culture of Animal Cells (eBook)

R. IAN FRESHNEY, PhD, is an honorary Senior Research Fellow at the Instituteof Cancer Sciences at the University of Glasgow in Glasgow, UK. He is the author or editor of numerous books and a world-renowned expert on cell culture techniques.

List of Figures, xix

List of Color Plates, xxiii

List of Tables, xxv

List of Protocols, xxvii

List of Minireviews, xxix

Preface and Acknlowledgments, xxxi

Abbreviations, xxxiii

About the Companion Website xxxviii

1. Introduction, 1

2. Biology of Cultured Cells, 19

3. Laboratory Design and Layout, 37

4. Equipment and Materials, 51

5. Aseptic Technique, 73

6. Safety, Bioethics, and Validation, 89

7. Culture Vessels and Substrates, 111

8. Defined Media and Supplements, 125

9. Serum-Free Media, 149

10. Preparation and Sterilization, 173

10. Supplementary Material* 11. Primary Culture, 207

11. Supplementary Material* 12. Subculture and Cell Lines, 235

13. Authentication and Validation, 259

14. Microbial Contamination, 289

15. Cryopreservation and Banking, 307

16. Cloning and Selection, 327

16. Supplementary Material* 17. Cell Sorting, 347

18. Cell Line Characterization, 359

19. Differentiation, 383

20. Three?]Dimensional Culture 401

21. Scale?]up and Automation, 427

22. Senescence, Immortalization, and Transformation, 455

23. Quantitation, 483

23 Supplementary Material* 24. Cytotoxicity, 513

24. Supplementary Material* 25. Culture of Specific Cell Types, 531

25. Supplementary Material* 26. Stem Cells, 571

26. Supplementary Material* 27. Training Programs, 591

28. Problem Solving 627

29. In Conclusion, 651

Supplementary Material* Appendix III: Sources of Equipment and Material

Appendix IV: Suppliers and Other Resource