Salamanders in Regeneration Research
Humana Press Inc. (Verlag)
978-1-4939-2494-3 (ISBN)
Authoritative and up-to-date, Salamanders in Regeneration Research: Methods and Protocols provides a comprehensive collection of methods chapters.
Variation in Salamanders: An Essay on Genomes, Development, and Evolution.- Maintaining Eastern Newts (Notophthalmus viridescens) for Regeneration Research.- Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl.- Husbandry of Spanish Ribbed Newts (Pleurodeles waltl.- Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies.- Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH.- Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation.- The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration.- High-efficiency Electroporation of the Spinal Cord in Larval Axolotl.- Pseudotyped Retroviruses for Infecting Axolotl.- Thyroxine Induced Metamorphosis in the Axolotl (Ambystoma mexicanum).- Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos.- In vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration.- Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas.- Culture and Transfection of Axolotl Cells.- Isolation and Culture of Neurospheres from the Adult Newt Brain.- Methods for Axolotl Blood Collection, Intravenous Injection and Efficient Leukocyte Isolation from Peripheral Blood and the Regenerating Limb.- Assessing Cardiomyocyte Proliferative Capacity in the Newt Heart and Primary Culture.- Long Term Organ Cultures of Newt Hearts.- In vitro Preparation of Newt Inner Ear Sensory Epithelia as a Model for Repair and Regeneration .- Transgenesis in Axolotl (Ambystoma mexicanum).- Generating and Identifying Axolotls with Targeted Mutations using Cas9 RNA‐guided Nuclease.- Gene Manipulation for Regenerative Studies using the Iberian Ribbed Newt, Pleurodeles waltl.- Transcriptomics Using Axolotls.- Sal-Site: Research Resources for The Mexican Axolotl.- Data Mining in Newt-omics, the Repository for Omics Data from the Newt.
Reihe/Serie | Methods in Molecular Biology ; 1290 |
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Zusatzinfo | 81 Illustrations, color; 8 Illustrations, black and white; X, 357 p. 89 illus., 81 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Naturwissenschaften ► Biologie ► Zoologie | |
Technik ► Umwelttechnik / Biotechnologie | |
Schlagworte | brain • Evolutionary • Genetically Modified Organisms • genomic databases • heart • in vitro • In vivo • phylogenetic • salamander regeneration • salamander species |
ISBN-10 | 1-4939-2494-X / 149392494X |
ISBN-13 | 978-1-4939-2494-3 / 9781493924943 |
Zustand | Neuware |
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