Far-Field Optical Nanoscopy

Stefan W. Hell is director at the Max Planck Institute for Biophysical Chemistry in Göttingen and leads the Department of NanoBiophotonics, as well as the High Resolution Optical Microscopy division at the German Cancer Research Center (DKFZ) in Heidelberg. He is honorary professor of experimental physics at the University of Göttingen and holds a position as adjunct professor of physics at the University of Heidelberg. Stefan Hell has invented the first method, which took optical microscopy into the nano-dimension, breaking Abbe’s diffraction-limited resolution barrier. This breakthrough has been honored with the 2014 Nobel Prize® in Chemistry, awarded jointly to Stefan W. Hell and his colleagues Eric Betzig and William E. Moerner. Hell received his PhD from the University of Heidelberg in 1990 and worked at the European Molecular Biology Laboratory Heidelberg (EMBL) from 1991 to 1993. Afterwards he worked as senior researcher at the University of Turku, Finland and visiting scientist at the University of Oxford, England, before moving to the Max Planck Institute for Biophysical Chemistry in Göttingen in 1997. After building up his research group at the institute, he was appointed as a director in 2002 and established the department of Nanobiophotonics at the institute.

Philip Tinnefeld is Professor of Biophysical Chemistry at the TU Braunschweig. He received his PhD in Physical Chemistry from the University of Heidelberg. Afterwards he did research at the Universities of California in Los Angeles, Heidelberg, Leuven, and Bielefeld. From 2007 to 2010 Tinnefeld was Professor of Biophysics at LMU München, before moving to Braunschweig, where he now leads the group of NanoBioSciences at the Institute of Physical and Theoretical Chemistry of the TU Braunschweig.

Christian Eggeling is Professor of Molecular Immunology at the University of Oxford and Scientific Director at the Wolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine. Eggeling received his PhD from the Georg-August University Göttingen. Afterwards he did postdoctoral research at the Karolinska-Institute in Stockholm, Sweden. From 2000 to 2004 Eggeling worked at the company Evotec OAI AG Hamburg, before joining the NanoBiophotonics department of Stefan Hell at the Max-Planck-Institute for Biophysical Chemistry in Göttingen. In 2012 he moved to Oxford as Group Leader of the MRC Human Immunology Unit (HIU).

Part I: Optical Nanoscopy Techniques
 STED Fluorescence Nanoscopy
Christian Eggeling, Stefan W. Hell
 Super-Resolution Imaging Through Stochastic Switching and Localization of Single Molecules: An Overview
Ke Xu, Sang-Hee Shim, and Xiaowei Zhuang
 A Practical Guide to dSTORM: Super-Resolution Imaging with Standard Fluorescent Probes
Markus Sauer
 Part II: Labelling Technology for Optical Nanoscopy
 Single-Molecule Photocontrol and Nanoscopy
Matthew D. Lew, Steven F. Lee, Michael A. Thompson, Hsiao-lu D. Lee, and W. E. Moerner
 Probes for Nanoscopy: Fluorescent Proteins
Susan Gayda, Per Niklas Hedde, Karin Nienhaus, and G. Ulrich Nienhaus
 Tailoring Fluorescent Labels for Far-Field Nanoscopy
Dmytro A. Yushchenko and Marcel P. Bruchez
 Probes for Nanoscopy: Photoswitchable Fluorophores
Pedro F. Aramendia and Mariano L. Bossi
 Far-Field Nanoscopy with Conventional Fluorophores: Photostability, Photophysics, and Transient Binding
Thorben Cordes, Jan Vogelsang, Christian Steinhauer, Ingo H. Stein, Carsten Forthmann, Andreas Gietl, Jürgen J. Schmied, Guillermo P. Acuna, Sebastian Laurien, Birka Lalkens, and Philip Tinnefeld
 Part III: Developments and Applications of Optical Nanoscopy
 NASCA Microscopy: Super-Resolution Mapping of Chemical Reaction Centers
Gert De Cremer, Bert F. Sels, Dirk E. De Vos, Johan Hofkens, and Maarten B.J. Roeffaers
 Counting Molecules: Toward Quantitative Imaging
Maximilian H. Ulbrich
 In Vivo Tracking of Single Biomolecules: What Trajectories Tell Us About the Acting Forces
Mario Brameshuber and Gerhard J. Schütz
 
 

"This is the first comprehensive book that summarizes most of the superresolution imaging techniques, and as such it is an excellent resource for newcomers to the field. ... a unique and much needed resource that contains a detailed description of all the superresolution techniques and methods that led to the award of the Nobel Prize in Chemistry in 2014. It is a great first resource for anyone wanting to learn more about this rapidly evolving field of research." (Thomas Huser, Analytical and Bioanalytical Chemistry, Vol. 408, 2016)