Chromosomal Mutagenesis

Genome Editing by Targeted Chromosomal Mutagenesis.- ­piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells.- Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy.- Genome Mutagenesis Using Synthetic Triplex-Forming Molecules and Recombinagenic DNA.- Genome Engineering Using Adeno-Associated Virus (AAV).- Engineering of Customized Meganucleases Via In Vitro Compartmentalization and In Cellulo Optimization.- Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform.- Ligation-Independent Cloning (LIC) Assembly of TALENs.- Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications.- Genome Engineering Using CRISPR-Cas9 System.- Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases.- Endogenous Gene Tagging with Fluorescent Proteins.- Silencing Long Non-Coding RNAs with Genome-Editing Tools.- Gene Editing Using ssODNs with Engineered Endonucleases.- Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases.- Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells.- Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models.- Creating Knockout and Knockin Rodents Using Engineered Endonucleases Via Direct Embryo Injection.- Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains.