Agrobacterium Protocols
Springer-Verlag New York Inc.
978-1-4939-1694-8 (ISBN)
Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.
Gateway® Compatible Plant Transformation Vectors.- Brachypodium Distachyon.- Medicago Truncatula Transformation Using Cotyledonary Explants.- Medicago Truncatula Transformation Using Leaf Explants.- Setaria Viridis.- Barley (Hordeum Vulgare L.) Transformation Using Immature Embryos.- Barley (Hordeum Vulgare L.) Transformation Using Embryogenic Pollen Cultures.- Maize (Zea Mays L.).- Maize, Tropical (Zea Mays L.). - Finger Millet [Eleusine Coracana (L.) Gaertn].- Oat (Avena Sativa L.).- Rice, Indica (Oryza Sativa L.).- Rice, Japonica (Oryza Sativa L.).- Sorghum (Sorghum Bicolor L.).- Wheat (Triticum Aestivum L.) Transformation Using Immature Embryos.- Wheat (Triticum Aestivum L.) Transformation Using Mature Embryos.- Alfalfa (Medicago Sativa L.).- Clovers (Trifolium Spp.).- Clover, Red (Trifolium Pratense).- Cowpea [Vigna Unguiculata (L.) Walp].- Lentil (Lens Culinaris Medik.).- Soybean [Glycine Max (L.) Merr.].- Brassica Oleracea And B. Napus.- Cucumber (Cucumis Sativus L.) and Kabocha Squash (Cucurbita Moschata Duch).- Pepper, Chili (Capsicum Annuum).- Pepper, Chili (Capsicum Annuum).- Pepper, Sweet (Capsicum Annuum).- Sugar Beet (Beta Vulgaris L.).- Tomato (Solanum Lycopersicum).
Reihe/Serie | Methods in Molecular Biology ; 1223 |
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Zusatzinfo | 29 Illustrations, color; 13 Illustrations, black and white; XIV, 368 p. 42 illus., 29 illus. in color. |
Verlagsort | New York |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Botanik | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Technik ► Umwelttechnik / Biotechnologie | |
Schlagworte | agricultural crops • Agrobacterium-mediated transformation • Barley • biolistic gun • Citrus • DNA molecules • Electroporation • integrated transgenes • Plant Cells • plant materials • Plant species • Rice • Wheat |
ISBN-10 | 1-4939-1694-7 / 1493916947 |
ISBN-13 | 978-1-4939-1694-8 / 9781493916948 |
Zustand | Neuware |
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