Essential Molecular Biology -

Essential Molecular Biology

Terry Brown (Herausgeber)

Buch | Hardcover
310 Seiten
2001 | 2nd Revised edition
Oxford University Press (Verlag)
978-0-19-963645-7 (ISBN)
208,85 inkl. MwSt
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In Volume 2 of Essential Molecular Biology, procedures for preparing gene libraries and identifying genes are described, together with methods for studying the structure of a cloned gene and the way it is expressed in the cell.
The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications
to suit personal requirements.

Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints.

1. Strategies for research in molecular biology ; 2. Construction of genomic libraries in lambda and cosmic vectors ; 3. Construction of a cDNA library ; 4. Nucleic acid labelling and detection ; 5. Immobilization of nucleic acids and hybridization analysis ; 6. DNA sequencing ; 7. The polymerase chain reaction ; 8. Protein expression in Escherichia coli ; 9. Transcript mapping ; APPENDICES ; 1. Health hazards and safety procedures ; 2. Equipment for molecular biology research ; 3. Important Escherichia coli strains ; 4. Recipes and general procedures ; 5. Restriction endonucleases ; 6. DNA and RNA modification enzymes ; 7. List of suppliers' addresses

Reihe/Serie Practical Approach Series ; 255
Zusatzinfo numerous line figures, 9 halftones
Verlagsort Oxford
Sprache englisch
Maße 194 x 253 mm
Gewicht 792 g
Themenwelt Studium 2. Studienabschnitt (Klinik) Humangenetik
Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Zellbiologie
Technik Lebensmitteltechnologie
ISBN-10 0-19-963645-1 / 0199636451
ISBN-13 978-0-19-963645-7 / 9780199636457
Zustand Neuware
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