Listeria monocytogenes

Sampling the Production/Processing Environment for Listeria.- Traditional Methods for Isolation of Listeria monocytogenes.- Confirmation of Isolates of Listeria by Conventional and Real-Time PCR.- Serotype Assignment by Sero-Agglutination, ELISA and PCR.- Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes.- Multi-Locus Sequence Typing (MLST) of Listeria monocytogenes.- Ribotyping and Automated Ribotyping of Listeria monocytogenes.- Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes.- High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray.- Analysis of Listeria monocytogenes Subproteomes.- The Listeria Cell Wall and Associated Carbohydrate Polymers.- Use of Bacteriophage Cell Wall Binding Proteins for Rapid Diagnostics of Listeria.- Virulence Characterization of Listeria monocytogenes.- Internalization Assays for Listeria monocytogenes.- Extraction and Analysis of Plasmid DNA from Listeria monocytogenes.- Generation of Non-Polar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method.- Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes.- Absolute and Relative Gene Expression in Listeria monocytogenes Using Real Time PCR.- Genome Sequencing of Listeria monocytogenes.- Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels.- Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers.- Vaccination Studies: Detection of a Listeria monocytogenes Specific T Cell Immune Response Using the ELISPOT Technique.- Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in FoodProcessing Environments.