Protein-Dye Interactions: Developments and Applications

Introduction: A Short Historical Review on the Interaction Between Dye-stuffs and Biological Matter.- Introduction: A Short Historical Review on the Interaction Between Dye-stuffs and Biological Matter.- 1: Dye Structures and their Relevance to Protein Recognition.- Biomimetic Dyes in Biotechnology.- Structure, Preparation and Chemistry of Reactive Dyes.- Dye Fragments in Protein—Dye Interactions.- 2: Physico-chemical Aspects of Dye—Protein Interactions.- Reactive Dyes as a Spectral Probe for Protein Folding.- Ionic and Apolar Interactions of Cibacron Blue F3GA with Model Compounds and Proteins: Application to Protein Purification.- A Quantitative Study of the Interaction of Lactate Dehydrogenase and Immobilised Cibacron Blue F3GA in the Absence of Soluble Ligands.- Interaction of Azo Dyes with Cationic Polypeptides: Poly-l-Arginine—4-Dimethylamino Azobenzene-4’—Carboxylic Acid System.- Kinetic and Thermodynamic of the Interaction of Dyes with Biological Macromolecules.- 3: Dye—Ligand Adsorbents in Chromatographic Systems.- The Use of Biospecific Elution in Purifying Proteins from Dye—Ligand Adsorbents.- The Influence of Matrices on Dye—Protein Interaction.- Evaluation of Chromatography Supports Prepared by Grafting Reactive Dyes onto Dextran Coated Silica Beads.- Fast Analytical Dye—Ligand Chromatography in Process Development.- A Comparison of Macrosorb—Cibacron Blue under Fixed-Bed, Batch Adsorption and Fluidised-Bed Conditions.- Polymer Design in Dye Chromatography: From the Definition of Monomers to the Evaluation of Polymeric Supports.- The Use of Dyes in Affinity Ligand and Protein Immobilization.- 4: Dye Ligands in Affinity Partition, Affinity Ultrafiltration and Affinity Precipitation.- Dye—Ligand Affinity Partitioning—a Powerful Methodfor Studying Enzyme—Dye Interaction.- Affinity Partition and Solubilisation of Enzymes by Use of Polymer-Bound Triazine Dyes.- The Comparative Study of the Interaction of Yeast and Horse Liver Alcohol Dehydrogenases with Reactive Dyes by Affinity Partitioning in Two-Phase Systems.- Affinity Ultrafiltration and Affinity Precipitation Using a Water-Soluble Complex of Poly(Vinyl Alcohol) and Cibacron Blue F3GA.- Affinity Precipitation of (NAD Dependent Dehydrogenase) Lactate Dehydrogenase Using Cibacron Blue Dimers.- 5: Role of Added Ions in Dye—Protein Interactions.- Metal Enhanced Interactions of Proteins with Triazine Dyes and Applications to Downstream Processing.- Metal Mediated Modulation of Enzyme Kinetics by Structurally Related Dyes.- Study of the Interaction of NAD(H)-Dependent Dehydrogenases with Reactive Dyes and their Complexes with Transition Metal Ions.- Immunoglobulin and Prealbumin Interactions with Remazol Yellow GGL: Prealbumin Requires an Anion.- 6: Blood Proteins Purification Using Dye—Ligand Affinity.- Dye Serum Protein Interaction—Analysis and Application.- Recovery of Albumin from Cohn Fraction IV Using Immobilised Procion Blue-HB: Development of a Full Scale Production Process.- The Interaction of Human ?-Fetoprotein with Reactive Dyes and the Isolation of the Protein by Affinity Chromatography.- 7: Dye—Ligand Affinity Chromatography for the Purification of Plant Proteins.- Downstream Processing of Potato Proteins.- A New Procedure for Purification of Pop Corn Inhibitor Based on Immobilized Dye Chromatography.- 8: Dyes in Molecular Biology.- Influence of Dye Binding on the Integrity and Function of Nucleic Acids and Nucleoproteins: Complexation of Polysomes with Coomassie Blue.- Progressive Unfolding of the Conformational States ofTransfer RNA and Ribosomal 5S RNA by Methylene Blue Binding.- Use of Triazine Dyes in Purification of T4 Polynucleotide Kinase.- Epilogue.- Index of Contributors.