Fundamentals of Light Microscopy and Electronic Imaging (eBook)

Douglas B. Murphy joined The Johns Hopkins University School of Medicine in 1978, where he presently holds the position of professor of cell biology. Dr. Murphy's research on cell motility and the assembly and dynamics of cytoskeletal polymers involves extensive use of light and electron microscopy, and for the past 20 years he has taught courses for students and faculty on light microscopy, confocal fluorescence microscopy and EM. He is co-founder and director of an institution-wide School of Medicine Microscope Facility that provides microscope services to over 100 research laboratories.

Preface.

Fundamentals of Light Microscopy.

Light and Color.

Illuminators, Filters, and Isolation of Specific Wavelengths.

Lenses and Geometrical Optics.

Diffraction and Interference in Image Formation.

Diffraction and Spatial Resolution.

Phase Contrast Microscopy and Dark-Field Microscopy.

Properties of Polarized Light.

Polarization Microscopy.

Differential Interference Contrast (DIC) Microscopy and Modulation Contrast Microscopy.

Fluorescence Microscopy.

Confocal Laser Scanning Microscopy.

Video Microscopy.

Digital CCD Microscopy.

Digital Image Processing.

Image Processing for Scientific Publication.

Appendix I.

Appendix II.

Appendix III.

Glossary.

References.

Index.

"...will greatly advance your knowledge of practical light imaging techniques..." (IEEE Electrical Insulation Magazine, July/August 2007)

"...To conclude, it is an outstanding book!" (Fundamentals of Light Microscopy and Electronic Imaging Microscopy and Analysis, July 2002)

"Both theoretical and practical aspects of the subject are treated with economy, clarity, and authority....Murphy?s book is unusual because it provides a technically sound and simply conceived introduction to virtually every link in the chain of technologies that makes modern imaging so powerful....This book will provide individuals without background knowledge in optical physics, electronics, or image processing with many of the basic facts they need to know to understand both the power and the limitations of their images." --J. Richard McIntosh, Cell Biology Education"This well written text provides a clear, uncluttered overview of the principles and practice of modern light microscopy. It contains many helpful teaching exercises and diagrams. It should prove useful in a wide range of courses from the undergraduate to the postgraduate level." (Kenneth R. Spring, Ph.D., Author)

"Douglas Murphy's book, Fundamentals of Light Microscopy and Electronic Imaging, is a welcome tool to introduce students in the biological sciences to the fundamentals of modern light microscopy and its augmentation by electronic imaging methods"..."this book provides a comprehensive introduction to the range of light-based methods available today"..."Each section has illustrative exercises and/or demonstrations, making this book readily adaptable to course work. " (Gordon W. Ellis, University of Pennsylvania)

"Given the remarkable resurgence of interest in biological microscopy during the past few years and the genuine need for a text in this field, Murphy's book will be warmly welcomed by all cell biologists who use microscopes in their research." (Joseph G. Gall, American Cancer Society Professor of Developmental Genetics, Carnegie Institution)

"This well written text provides a clear, uncluttered overview of
the principles and practice of modern light microscopy. It contains
many helpful teaching exercises and diagrams. It should prove
useful in a wide range of courses from the undergraduate to the
postgraduate level." (Kenneth R. Spring, Ph.D., Author)

"Douglas Murphy's book, Fundamentals of Light Microscopy and
Electronic Imaging, is a welcome tool to introduce students in the
biological sciences to the fundamentals of modern light microscopy
and its augmentation by electronic imaging methods"..."this book
provides a comprehensive introduction to the range of light-based
methods available today"..."Each section has illustrative exercises
and/or demonstrations, making this book readily adaptable to course
work. " (Gordon W. Ellis, University of Pennsylvania)

"Given the remarkable resurgence of interest in biological
microscopy during the past few years and the genuine need for a
text in this field, Murphy's book will be warmly welcomed by all
cell biologists who use microscopes in their research." (Joseph G.
Gall, American Cancer Society Professor of Developmental Genetics,
Carnegie Institution)