Animal Cell Culture (eBook)

Dr John Davis: Visiting Lecturer in Biotechnology, University of Hertfordshire, previously Senior Project Scientist at Bio Products Laboratory, UK.

Contributors.

Preface.

Abbreviations.

Protocols.

1 The Cell Culture Laboratory (Sue Clarke and Janette
Dillon).

2 Sterilization (Peter L. Roberts).

3 Microscopy of Living Cells (Colin Gray and Daniel
Zicha).

4 Basic Techniques and Media, the Maintenance of Cell Lines, and
Safety (John M. Davis).

5 Development and Optimization of Serum- and Protein-free
Culture Media (Stephen F. Gorfien and David W. Jayme).

6 Cryopreservation and Banking of Cell Lines (Glyn N. Stacey,
Ross Hawkins and Roland A. Fleck).

7 Primary Culture of Specific Cell Types and the Establishment
of Cell Lines (Kee Woei Ng, Mohan Chothirakottu Abraham, David
Tai Wei Leong, Chris Morris and Jan-Thorsten Schantz).

8 Cloning (John Clarke, Alison Porter and John M.
Davis).

9 The Quality Control of Animal Cell Lines and the Prevention,
Detection and Cure of Contamination (Peter Thraves and Cathy
Rowe).

10 Systems for Cell Culture Scale-up (Jennifer Halsall and
John M. Davis).

11 Good Laboratory Practice in the Cell Culture Laboratory
(Barbara Orton).

Index.

Colour plates.

"It will be a great resource for students and established researchers who want to learn more about modern cell culture techniques." (Doody's, 13 January 2012)