Multidimensional Liquid Chromatography (eBook)

Steven A. Cohen, PhD, is Life Sciences Director, RDE of the Proteomics Technology Group at the Waters Corporation in Milford, Massachusetts. MARK R. SCHURE, PhD, is Technical Director of the Computational Chemistry Group and Technical Director of the Theoretical Separation Science Laboratory at the Rohm and Haas Company in Springhouse, Pennsylvania.

Preface.

1. Introduction, (Mark R. Schure and Steven A. Cohen).

Theory.

2. Elements of the Theory of Multidimensional LiquidChromatography, (Mark R. Schure).

3. Peak capacity in Two-dimensional Liquid Chromatography (JoeM. Davis).

4. Decoding Complex 2-D Separations (Francseso Dondi, MariaChiara Pietrogrande, Nicola Marchetti and Attila Felinger).

Columns, Instrumentation, and Methods.

5. Instrumentation for Comprehensive Multidimensional LiquidChromatography (Robert. E. Murphy and Mark R. Schure).

6. Methods Development for Comprehensive Multidimensional LiquidChromatography (Robert E. Murphy and Mark R. Schure).

7. Monolithic Columns and their 2D-HPLC applications (TohruIkegami, Hiroshi Aoki, Hiroshi Kimura, Ken Hosoya and NobuoTanaka).

8. Ultra-High Pressure Multidimensional Liquid Chromatography(Charles R. Evans and James W. Jorgenson).

Life Sciences Applications.

9. Peptidomics (Egidijus Machtejevas, Klaus K. Unger).

10. A Two-dimensional Liquid Mass Mapping Technique forBiomarker (David Lubman, Nathan S. Buchanan, Fred R. Miller,Kathleen Cho, Rong Wu, Steven Goodison, Yanfei Wang, PaweenaKreunin and Timothy J. Barder).

11. Coupled Multidimensional Chromatography and Tandem MassSpectrometry Systems for Complex Peptide Mixture Analysis (MikeWashburn).

12. Development of Orthogonal 2D-LC Methods for Separations ofPeptides (Martin Gilar, Petra Olivova, Amy E. Daly and John C.Gebler).

13. Protein IEX-RP-TOF MS (Steven A. Cohen and ScottBerger).

14. Analysis of Enantiomeric Compounds Using MultidimensionalLiquid Chromatography (Renee J. Soukup and Dan Armstrong).

Multidimensional separations using CE.

15. Two-dimensional capillary electrophoresis for thecomprehensive analysis of complex protein mixtures (James R. Kraly,Melissa M. Harwood, Megan Jones and Norman J. Dovici).

16. Two-dimensional HPLC-CE methods for protein/Peptideseparation (Haleem Issaq).

Industrial Applications.

17. Multidimensional Liquid Chromatography in IndustrialApplications (Frank Rittig and Harald Pasch).

18. The Analysis of Surfactants by Multidimensional LiquidChromatography (Robert E. Murphy and Mark R. Schure.

"It is a timely publication and present a valuable resource of scientific information on MDLC...It presents systematically gathered scientific information from a plethora of articles scattered over a wide range of sources. This effort should be appreciated by a wide audience of scientists and researchers who deal with complex separation programs in biomedical, environmental, and natural products; industrial polymers; food and other sources." (Journal of the American Chemical Society, November 12, 2008)