Practical HPLC Method Development (eBook)

Lloyd R. Snyder, PHD, is a Principal at LC Resources in Walnut Creek, California. He is the author or coauthor of several books including An Introduction to Separation Science, Introduction to Modern Liquid Chromatography, Second Edition, and the bestselling Practical HPLC Method Development, Second Edition, all published by Wiley. Joseph J. Kirkland, PhD, is Vice President of Research and Development for Advanced Materials Technology, Inc., and coauthor of Introduction to Modern Liquid Chromatography, Second Edition, Practical HPLC Method Development, Second Edition, and Modern Size-Exclusion Liquid Chromatography, Second Edition, all published by Wiley. Joseph L. Glajch is the author of Practical HPLC Method Development, Second Edition, published by Wiley.

Getting Started.

Basics of Separation.

Detection Sensitivity and Selectivity.

Sample Preparation.

The Column.

Non-Ionic Samples: Reversed- and Normal-Phase HPLC.

Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.

Gradient Elution.

Systematic Approach to the Reversed-Phase Separation of Regular Samples.

Computer-Assisted Method Development.

Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.

Chiral Separations.

Preparative HPLC Separation.

Quantitation (Including Trace Analysis).

Completing the Method: Validation and Transfer.

Appendices.

Index.