Hydrophilic Interaction Chromatography (eBook)
336 Seiten
Wiley (Verlag)
978-1-118-49521-6 (ISBN)
BERNARD A. OLSEN, PhD, has close to three decades of experience at Eli Lilly and Company in chemistry, manufacturing, and control of drug substances and drug products. He contributed to the development and support of more than twenty-five commercialized drugs as well as numerous developmental drugs. A Fellow of the American Association of Pharmaceutical Scientists, Dr. Olsen currently provides consulting and training services to the pharmaceutical industry and serves as Chair of the USP Expert Committee on Monograph Development: Small Molecules 3. BRIAN W. PACK, PhD, is a Research Advisor in Analytical Sciences R&D at Eli Lilly and Company, where he has contributed to the development of many solid oral and parenteral dosage forms. He is recognized for his contributions to HPLC method development, raw material control strategies, genotoxic impurities, cleaning validation, and colorimetry. Dr. Pack has published on the topics of HILIC applications, Raman spectroscopy, mass spectrometry, dissolution, and cleaning verification.
PREFACE xiii
CONTRIBUTORS xv
CHAPTER 1 SEPARATION MECHANISMS IN HYDROPHILIC INTERACTION CHROMATOGRAPHY 1
David V. McCalley
1.1 Introduction 1
1.2 Historical Background: Recognition of the Contribution of Partition, Ion Exchange, and RP Interactions to the Retention Process 3
1.3 Recent Studies on the Contributory Mechanisms to HILIC Retention 7
1.4 Conclusions 38
References 38
CHAPTER 2 STATIONARY PHASES FOR HILIC 43
Mohammed E.A. Ibrahim and Charles A. Lucy
2.1 Introduction 43
2.2 HILIC Stationary Phases 44
2.3 Commercial HILIC Phases 61
2.4 Conclusions 77
Acknowledgments 77
References 77
CHAPTER 3 HILIC METHOD DEVELOPMENT 87
Yong Guo and Xiande Wang
3.1 Introduction 87
3.2 General Method Development Considerations 88
3.3 HILIC Method Development 93
3.4 Detection for HILIC Methods 104
3.5 Conclusions 107
References 108
CHAPTER 4 PHARMACEUTICAL APPLICATIONS OF HYDROPHILIC INTERACTION CHROMATOGRAPHY 111
Bernard A. Olsen, Donald S. Risley, V. Scott Sharp, Brian W. Pack, and Michelle L. Lytle
4.1 Introduction 112
4.2 Determination of Counterions 117
4.3 Main Component Methods 129
4.4 Determination of Impurities 135
4.5 Excipients 146
4.6 Chiral Applications 152
4.7 Conclusions 161
References 161
CHAPTER 5 HYDROPHILIC INTERACTION CHROMATOGRAPHY (HILIC) FOR DRUG DISCOVERY 169
Alfonso Espada and Mark Strege
5.1 Drug Discovery Model 169
5.2 HILIC Applications for In Vitro Biology 170
5.4 Practical Considerations 186
5.5 Conclusions 187
References 188
CHAPTER 6 ADVANCES IN HYDROPHILIC INTERACTION CHROMATOGRAPHY (HILIC) FOR BIOCHEMICAL APPLICATIONS 195
Fred Rabel and Bernard A. Olsen
6.1 Introduction 195
6.2 Carbohydrates 196
6.2.1 Mono- and Disaccharides 196
6.3 Nucleobases and Nucleosides 203
6.4 Oligonucleotides 205
6.5 Amino Acids and Peptides 206
6.6 Proteins 209
6.7 Phospholipids 209
6.8 Conclusions 211
References 212
CHAPTER 7 HILIC-MS FOR TARGETED METABOLOMICS AND SMALL MOLECULE BIOANALYSIS 219
Hien P. Nguyen, Heather D. Tippens, and Kevin A. Schug
7.1 Introduction 219
7.2 The Role of HILIC-MS in Targeted Metabolomics versus Other LC Modes 221
7.3 Strategies for Method Development Based on Retention Behavior of Targeted Metabolites on HILIC Stationary Phases 223
7.4 Summary 231
Acknowledgments 232
References 232
CHAPTER 8 HILIC FOR FOOD, ENVIRONMENTAL, AND OTHER APPLICATIONS 239
Michael A. Koupparis, Nikolaos C. Megoulas, and Aikaterini M. Gremilogianni
8.1 Introduction 239
8.2 Food Applications for HILIC 240
8.3 Environmental and Other Applications of HILIC 254
8.4 Conclusions 257
References 259
CHAPTER 9 THEORY AND PRACTICE OF TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY SEPARATIONS INVOLVING THE HILIC MODE OF SEPARATION 265
Stephen R. Groskreutz and Dwight R. Stoll
9.1 Fundamentals of Multidimensional Liquid Chromatography 265
9.2 Complementarity of HILIC Selectivity to Other Separation Modes 278
9.3 Instrumentation and Experimental Considerations 278
9.4 Applications 291
9.5 The Future of HILIC Separations in 2DLC 298
References 298
INDEX 307
"The well-balanced mixture with updated HILIC theory and
detailed practical descriptions makes it a useful resource for
scientists working in analytical chemistry, biochemistry,
biotechnology, and bioinformatics, and a valuable resource for
people in the analytical column industry."
(Analytical and Bioanalytical Chemistry, 22 September
2013)
Erscheint lt. Verlag | 14.1.2013 |
---|---|
Reihe/Serie | Chemical Analysis: A Series of Monographs on Analytical Chemistry and Its Applications | Chemical Analysis: A Series of Monographs on Analytical Chemistry and Its Applications |
Mitarbeit |
Herausgeber (Serie): Mark F. Vitha |
Sprache | englisch |
Themenwelt | Naturwissenschaften ► Chemie ► Analytische Chemie |
Technik | |
Schlagworte | Biowissenschaften • Chemie • Chemistry • Chromatographie • Chromatographie / Trennverfahren • Chromatography / Separation Techniques • Drug Discovery & Development • Genomforschung u. Proteomik • Genomics & Proteomics • Life Sciences • Wirkstoffforschung • Wirkstoffforschung u. -entwicklung |
ISBN-10 | 1-118-49521-7 / 1118495217 |
ISBN-13 | 978-1-118-49521-6 / 9781118495216 |
Haben Sie eine Frage zum Produkt? |
Größe: 11,6 MB
Kopierschutz: Adobe-DRM
Adobe-DRM ist ein Kopierschutz, der das eBook vor Mißbrauch schützen soll. Dabei wird das eBook bereits beim Download auf Ihre persönliche Adobe-ID autorisiert. Lesen können Sie das eBook dann nur auf den Geräten, welche ebenfalls auf Ihre Adobe-ID registriert sind.
Details zum Adobe-DRM
Dateiformat: PDF (Portable Document Format)
Mit einem festen Seitenlayout eignet sich die PDF besonders für Fachbücher mit Spalten, Tabellen und Abbildungen. Eine PDF kann auf fast allen Geräten angezeigt werden, ist aber für kleine Displays (Smartphone, eReader) nur eingeschränkt geeignet.
Systemvoraussetzungen:
PC/Mac: Mit einem PC oder Mac können Sie dieses eBook lesen. Sie benötigen eine
eReader: Dieses eBook kann mit (fast) allen eBook-Readern gelesen werden. Mit dem amazon-Kindle ist es aber nicht kompatibel.
Smartphone/Tablet: Egal ob Apple oder Android, dieses eBook können Sie lesen. Sie benötigen eine
Geräteliste und zusätzliche Hinweise
Buying eBooks from abroad
For tax law reasons we can sell eBooks just within Germany and Switzerland. Regrettably we cannot fulfill eBook-orders from other countries.
aus dem Bereich