Mass Spectrometry for Microbial Proteomics (eBook)

Professor Haroun N. Shah is Head of Molecular Identification Services Unit at the Centre for Infections, Health Protection Agency, London. The Centre for Infections provides infectious disease surveillance and microbial identification services, co-ordinating the investigation and cause of national and uncommon outbreaks of diseases. Haroun holds several chairs at various universities and spent 25 years in academic life at the University of London. Professor Saheer E. Gharbia is Head of the Applied and Functional Genomics Unit at the HPA Centre for Infections. She has a PhD in molecular Genetics and Biochemistry, and has held various academic positions at the University of London and McGill University, Canada.

Section 1: Microbial Characterisation; the Transition from conventional methods to Proteomics.

(1) Changing concepts in the characterisation of microbes - H. N. Shah (HPA).

(2) Microbial phylogeny and evolution; the change from targeted genes to proteins - Radhey Gupta (Canada),.

.

Section 2: Mass Spectrometers and Specific Applications.

(3) Range of instruments; criteria for selection for microbial analysis. - Robert Parker (HPA).

(4) Tools for rapid Mass spectral analysis (Bob Galvin (ABI).

(5) Hybrid Mass Spectrometers - Michaela Scigelova (Thermo).

Section 3: Protein Samples Preparation Techniques.

(6) Conventional approaches for sample preparation and 2-D gel electrophoresis applications: Vesela Encheva (HPA).

(7) Protein fractionation and analysis using multidimensional chromatography (PF2D).- Jim Thorn, BeckmanCoulter Inc.

(8) On-Chip sample preparation and concentration using newly developed surfaces prior to high-sensitivity MALDI analysis. Winfred Girg (Qiagen).

(9) News affinity capture methods: David O'Connor (Univ. Southampton)..

Section 4: Characterisation of microorganisms by Pattern Matching of Mass Spectral Profiles and Biomarker Approaches Requiring Minimal Sample Preparation.

(10) Analysis of Surface-Associated Molecules of Intact Bacterial Cells using.

MALDI- TOF-MS - Diane Dare.

(11) Analysis based upon Membrane-Bound/Intracellular Protein Patterns using.

SELDI-TOF-MS - H. N. Shah et al.(MISU).

(12) Microbial Disease Biomrakers using ProteinChip Arrays -Paul Langford,.

Imperial.

(13) Rapid identification of microorganisms using stable intracellular proteins.

Markus Kostrzewa & Wolfgang Pusch, Brucker, Germany.

*Plus additional chapter on viruses (Catherine Fenselau to be invited).

Section 5: Targeted Molecules and Analysis of Specific Microorganisms.

(14) Analysis of High risk pathogens - Ben van Baar (Holland).

(15) Proteome Analysis of E. coli - Robin Wait (Imperial College).

(16) Comparative DNA sequence analysis and typing using Mass Spectrometry.

- Christiane Honisch & Yong Chen; SEQUENOM Inc. (USA).

(17) Analysis based upon whole cell digestion; comparative proteomic of.

antibiotic resistant and sensitive strains of Staphylococcus aureus. Therese.

McKenna et al.

Section 6: Data Analysis.

(18) Artificial Neural Networks of Mass Spectral Data - Graham Ball (Nottingham Trent University).

(19) Devising a platform for microbial identification and comparative.

analysis: Martin Lund (Waters).

(20) Analysis of 2-D gels using DeCyder EDA (Extended Data Analysis).

Matthias Berg, Germany.

* Plus two additional chapters from Jerry Thomas (University of York) and Natasha Karp (University of Cambridge)

"Summary Mass Spectrometry of Microbial Proteomics
provides an authoritative guide to the expanding field of microbial
proteomics." (Anal Bioanal Chem, 2011)