Practical Cell Analysis (eBook)

As analytical chemistry and biology move closer together, biologists are performing increasingly sophisticated analytical techniques on cells. Chemists are also turning to cells as a relevant and important sample to study newly developed methods. Practical Cell Analysis provides techniques, hints, and time-saving tips explaining what may be 'common knowledge' to one field but are often hidden or unknown to another.

Within this practical guide:

• The procedures and protocols for cell separation, handling cells on a microscope and for using cells in microfluidic devices are presented.
• Elements of cell culture are taken and combined with the practical advice necessary to maintain a cell lab and to handle cells properly during an analysis
• The main chapters deal with the fundamentals and applied aspects of each technique, with one complete chapter focusing on statistical considerations of analyzing cells
• Many diagram-based protocols for some of the more common cell processes are included
• Chapter summaries and extensive tables are included so that key information can be looked up easily in the lab setting

Much like a good manual or cookbook this book is a useful, practical guide and a handy reference for all students, researchers and practitioners involved in cellular analysis.

Dimitri Pappas is Assistant Professor in Analytical Chemistry at Texas Tech University. He obtained his B.S., University of Florida, 1998 followed by his Ph.D., University of Florida, 2002. He worked as a Research Scientist at Wyle Laboratories, NASA/Johnson Space Center, 2002-05 before taking up his post at Texas Tech. His key areas of research are: Analytical Fluorescence and Raman Spectroscopy; Cellular Analysis; Flow Cytometry; Optics
With the focus on the interface between cell science and analytical chemistry. Dr. Pappas is currently developing novel analytical methods to study intact cells, cell digests, and tissue aggregates using spectroscopic and immunochemical techniques. The majority of his group's research is geared toward clinical analysis as well as medical testing in remote areas.

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As analytical chemistry and biology move closer together, biologists are performing increasingly sophisticated analytical techniques on cells. Chemists are also turning to cells as a relevant and important sample to study newly developed methods. Practical Cell Analysis provides techniques, hints, and time-saving tips explaining what may be common knowledge to one field but are often hidden or unknown to another. Within this practical guide: The procedures and protocols for cell separation, handling cells on a microscope and for using cells in microfluidic devices are presented. Elements of cell culture are taken and combined with the practical advice necessary to maintain a cell lab and to handle cells properly during an analysis The main chapters deal with the fundamentals and applied aspects of each technique, with one complete chapter focusing on statistical considerations of analyzing cells Many diagram-based protocols for some of the more common cell processes are included Chapter summaries and extensive tables are included so that key information can be looked up easily in the lab setting Much like a good manual or cookbook this book is a useful, practical guide and a handy reference for all students, researchers and practitioners involved in cellular analysis.