Engineering Complex Phenotypes in Industrial Strains (eBook)

RANJAN PATNAIK, PhD, is a chemical engineer by profession with 17 years of practical experience in the field of industrial biotechnology that includes small biotech start-ups and large enterprises. His research interests are in the field of metabolic engineering, fermentation optimization, process integration and R&D technology management. He currently heads the biofuels research group at DuPont Knowledge Center (DKC), Hyderabad, India. Prior to his current role, he had a variety of responsibilities within the biochemical sciences & engineering division of CR&D, E. I. du Pont de Nemours and Company, Wilmington, DE, USA.

Foreword vii

John Pierce

Preface ix

Contributors xi

1 Classical Strain Improvement 1

Nathan Crook and Hal S. Alper

2 Tracer-Based Analysis of Metabolic Flux Networks 35

Michael Dauner

3 Integration of "Omics" Data with Genome-Scale
Metabolic Models 77

Stephen Van Dien, Priti Pharkya, and Robin Osterhout

4 Strain Improvement via Evolutionary Engineering 111

Byoungjin Kim, Jing Du, and Huimin Zhao

5 Rapid Fermentation Process Development and Optimization
133

Jun Sun and Lawrence Chew

6 The Clavulanic Acid Strain Improvement Program at DSM
Anti-Infectives 169

Bert Koekman and Marcus Hans

7 Metabolic Engineering of Recombinant E. coli for the
Production of 3-Hydroxypropionate 185

Tanya Warnecke Lipscomb, Matthew L. Lipscomb, Ryan T. Gill, and
Michael D. Lynch

8 Complex System Engineering: A Case Study for an Unsequenced
Microalga 201

Michael T. Guarnieri, Lieve M.L. Laurens, Eric P. Knoshaug,
Yat-Chen Chou, Bryon S. Donohoe, and Philip T. Pienkos

9 Meiotic Recombination-Based Genome Shuffling of Saccharomyces
Cerevisiae and Schefferomyces Stiptis for Increased Inhibitor
Tolerance to Lignocellulosic Substrate Toxicity 233

Dominic Pinel and Vincent J.J. Martin

Index 251

"This book should be valuable to engineers, biochemists,
and students who seek to understand the science and practice
of engineering biocatalysts for industrial
applications." (Chemical Engineering
Progress, 1 January 2013)