Enzymes in Industry (eBook)

Wolfgang Aehle is a senior scientist for Protein Engineering at Genencor International, a Danisco Company. After finishing his Ph.D. in Organic Chemistry, he discovered the beauty and excitement of working with protein 3D-structures during his postdoc period on molecular modeling of Subtilisin at the GBF in Braunschweig (Germany). Aehle further pursued his career in industry as a protein engineer. He has focused his work on a significant number of industrial enzymes, which are subject of this book. His research interest is driven by the wish to understand the enzyme function in the various industrial applications, whose physical conditions are far from the original environment of the enzymes. The main goal of his work is to improve enzyme function in the industrial environment. His work is documented in numerous scientific publications and patent applications.

Introduction: History, Nomenclature, Structure, Enzymes and Molecular Biology
CATALYTIC ACTIVITY OF ENZYMES
Factors Governing Catalytic Activity
Enzyme Assays
Quality Evaluation of Enzyme Preparations
GENERAL PRODUCTION METHODS
Fermentation
Isolation and Purification
Immobilization
INDUSTRIAL USES OF ENZYMES
Survey of Industrial Enzymes
Enzymes in Starch Processing and Baking
Glucose Isomerization
Proteolytic Enzymes
Meat Processing
Dairy Products
Processing of Fruit, Vegetables, and Wine
Hydrolysis of Protein, Fat, and Cellulose, and Inversion of Sucrose
Amino Acids and Hydroxycarboxylic Acids
Enzymes in Organic Synthesis
ENZYMES IN ANALYSIS AND MEDICINE
Survey
Enzymes in Diagnosis
Enzymes for Food Analysis
Enzymes in Therapy
Enzymes in Genetic Engineering: Restriction Endonucleases and Methylases
DNA Polymerases
RNA Polymerases
DNA Nucleases
RNA Nucleases
Modifying Enzymes
ECONOMIC ASPECTS
SAFETY AND ENVIRONMENTAL ASPECTS
References
Index
Introduction: History, Nomenclature, Structure, Enzymes and Molecular Biology
CATALYTIC ACTIVITY OF ENZYMES
Factors Governing Catalytic Activity
Enzyme Assays
Quality Evaluation of Enzyme Preparations
GENERAL PRODUCTION METHODS
Fermentation
Isolation and Purification
Immobilization
INDUSTRIAL USES OF ENZYMES
Survey of Industrial Enzymes
Enzymes in Starch Processing and Baking
Glucose Isomerization
Proteolytic Enzymes
Meat Processing
Dairy Products
Processing of Fruit, Vegetables, and Wine
Hydrolysis of Protein, Fat, and Cellulose, and Inversion of Sucrose
Amino Acids and Hydroxycarboxylic Acids
Enzymes in Organic Synthesis
ENZYMES IN ANALYSIS AND MEDICINE
Survey
Enzymes in Diagnosis
Enzymes for Food Analysis
Enzymes in Therapy
Enzymes in Genetic Engineering: Restriction Endonucleases and Methylases
DNA Polymerases
RNA Polymerases
DNA Nucleases
RNA Nucleases
Modifying Enzymes
ECONOMIC ASPECTS
SAFETY AND ENVIRONMENTAL ASPECTS
References
Index

'...The quality...is so great that there is no hesitation in recommending it as ideal reading for any student requiring an introduction to enzymes. ...Enzymes in Industry - should command a place in any library, industrial or academic, where it will be frequently used.'
The Genetic Engineer and Biotechnologist

'Enzymes in Industry' is an excellent introduction into the field of applied enzymology for the reader who is not familiar with the subject. ... offers a broad overview of the use of enzymes in industrial applications. It is up-to-date and remarkable easy to read, despite the fact that almost 50 different authors contributed. The scientist involved in enzyme work should have this book in his or her library. But it will also be of great value to the marketing expert interested in the present use of enzymes and their future in food and nonfood applications.'
Angewandte Chemie

'This book should be available to all of those working with, or aspiring to work with, enzymes. In particular academics should use this volume as a source book to ensure that their 'new' projects will not 'reinvent the wheel'.'
Journal of Chemical Technology and Biotechnology

'Die Qualität...ist so hervorragend, dass es uneingeschränkt als ideale Lektüre jedem Studenten, der eine Einführung in das Gebiet sucht, empfohlen werden kann. ...'Enzymes in Industry' sollte seinen Platz in jeder Bibliothek haben, sei es in der Industrie oder Hochschule, wo es mit Sicherheit oft seinen Nutzen finden wird.'
(The Genetic Engineer and Biotechnologist)

'Das Buch ist auf dem neuesten Stand und trotz der Beteiligung von fast 50 Autoren leicht zu lesen. ... Naturwissenschaftler, die sich mit Enzymen beschäftigen, sollten dieses Buch in ihrer Bibliothek haben. Das Buch wird aber auch für den Marketing-Spezialisten, der sich einen Überblick über gegenwärtige und künftige Anwendungen von Enzymen innerhalb und außerhalb der Lebensmittelindustrie machen will, von großem Nutzen sein.'
(Angewandte Chemie)

"Es gilt, die richtige Zellkultur zu optimieren, mit Fermenter-Limitierungen umzugehen sowie aufwendige Isolierungen bzw. Aufreinigungen durchzuführen. Das Buch liefert einen umfassenden Überblick dazu."
Chemiereport.at
Das Brachenmagazin Januar 2008