Carrier-bound Immobilized Enzymes (eBook)

Linqiu Cao studied Organic Chemistry and Biotechnology at the Beijing University of Science and Technology (China). He was a visiting scientist at GBF (Gesellschaft für Biotechnologische Forschung) in Braunschweig (Germany) and later moved to the University of Stuttgart (Germany) to obtain a PhD in Biotechnology. He then joined the group of Roger Sheldon at the Technical University in Delft (The Netherlands), where he pioneered a novel enzyme immobilisation technology with improved performance in biocatalytic transformations using cross-linked enzyme aggregates (CLEAs). In 2000, he joined the newly founded company Avantium in Amsterdam (The Netherlands) where he continued to develop novel enzyme crosslinking and immobilisation technology. Recently he moved to DMV in Veghel (The Netherlands).

IMMOBILIZED ENZYMES: PAST, PRESENT AND PROSPECTS
Introduction
The Past
Implications from the Past
Prospective and Future Development

ADSORPTION-BASED IMMOBILIZATION
Introduction and Classification
Principles of Adsorptive Enzyme Immobilization
Requirements of the Carriers
Factors which Dictate Enzyme Performance
Preparation of Immobilized Enzymes by Adsorption

COVALENT ENZYME IMMOBILIZATION
Introduction
Physical Nature of Carriers
Chemical Nature of Carriers
Amino Acid Residues for Covalent Binding of Enzymes
Factors Influencing Enzyme Performance
Preparation of Active Carriers

ENZYME ENTRAPMENT
Introduction and Definition
Requirements of the Carriers
Effect of the Entrapment
Preparation of Entrapped Enzymes

ENZYME ENCAPSULATION
Introduction and Classification
Effect of Encapsulation
Preparation of Encapsulated Enzymes

NON-CONVENTIONAL ENZYME IMMOBILIZATION
Coating-based Immobilization
Site-specific Immobilization
Immobilization in Organic Solvents
Imprinting Immobilization
Modification-based Immobilization
Post-immobilization Techniques

"The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. (...) A must for biotechnologists, biochemists and preparative chemists using enzymes in their daily work." Clinical Laboratory

"...the volume provides excellent and comprehensive review on the important field of enzyme immobilization technology covering both the history and present state of immobilization procedures. Numerous tables and figures throughout the volume provide illustrative material to support the detailed information presented in the text, and make this volume an excellent resource for biotechnologists, biochemists, biochemical engineers and enzyme technologists." Carbohydrate Polymers

"Es wird dazu beitragen, der rationalen Kombination der vorhandenen Prinzipien zur Enzymimmobilisierung die Zukunft zu ebnen."
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