Antisense Technology, Part A, General Methods, Methods of Delivery, and RNA Studies (eBook)

Cover 1
Table of Contents 6
Contributors to Volume 313 10
Preface 14
Volumes In Series 16
Section I: General Methods 35
Chapter 1. Progress in Antisense Technology: The End of the Beginning 37
Chapter 2. Basic Principles of Using Antisense Oligonucleotides in Vivo 80
Chapter 3. Polyethyleneimine-Mediated Transfection to Improve Antisense Activity of 3' -Capped Phosphodiester Oligonucleotides 90
Chapter 4. Design of Antisense and Triplex-Forming Oligonucleotides 108
Chapter 5. Chimeric Oligodeoxynucleotide Analogs: Chemical Synthesis, Purification, and Molecular and Cellular Biology Protocols 129
Chapter 6. Evaluation of Antisense Mechanisms of Action 169
Chapter 7. Physiology and Molecular Biology Brought to Single-Cell Level 177
Chapter 8. Antisense Properties of Peptide Nucleic Acid 190
Chapter 9. Synthesis of Oligonucleotide Conjugates in Anhydrous Dimethyl Sulfoxide 198
Chapter 10. Gene Switching: Analyzing Broad Range of Mutations Using Steric Block Antisense Oligonucleotides 208
Chapter 11. Vesicular Stomatitis Virus as Model System for Studies of Antisense Oligonucleotide Translation Arrest 223
Chapter 12. Purification of Antisense Oligonucleotides 237
Chapter 13. Boranophosphate Backbone: A Mimic of Phosphodiesters, Phosphorothioates, and Methyl Phosphonates 260
Chapter 14. Intracellular Distribution of Digoxigenin-Labeled Phosphorothiate Oligonucleotides 291
Chapter 15. Use of Minimally Modified Antisense Oligonucleotides for Specific Inhibition of Gene Expression 302
Section II: Methods of Delivery 319
Chapter 16. Determination of Cellular Internalization of Fluorescent Oligonucleotides 321
Chapter 17. Intrabody Tissue-Specific Delivery of Antisense Conjugates in Animals: Ligand–Linker Antisense Oligomer Conjugates 331
Chapter 18. Lipid-Based Formulations of Antisense Oligonucleotides for Systemic Delivery Applications 356
Chapter 19. In Vitro Transport and Delivery of Antisense Oligonucleotides 376
Chapter 20. In Vitro and in Vivo Delivery of Antisense Oligodeoxynucleotides Using Lipofection: Application of Antisense Technique to Growth Suppression of Experimental Glioma 393
Chapter 21. Preparation and Application of Liposome-Incorporated Oligodeoxynucleotides 406
Chapter 22. Cell-Specific Optimization of Phosphorothioate Antisense Oligodeoxynucleotide Delivery by Cationic Lipids 422
Section III: RNA Studies 433
Chapter 23. Intracellular Expression and Function of Antisense Catalytic RNAs 435
Chapter 24. Selective Degradation of Targeted mRNAs Using Partially Modified Oligonucleotides 454
Chapter 25. Reversible Depletion of Specific RNAs by Antisense Oligodeoxynucleotide-Targeted Degradation in Frog Oocytes 470
Chapter 26. Inactivation of Gene Expression Using Ribonuclease P and External Guide Sequences 476
Chapter 27. Disruption of mRNA-RNP Formation and Sorting to Dendritic Synapses by Antisense Oligonucleo- tides 490
Chapter 28. Antisense RNA and DNA in Escherichia coli 501
Chapter 29. Utilization of Properties of Natural Catalytic RNA to Design and Synthesize Functional Ribozymes 519
Chapter 30. Antisense Oligonucleotides and RNAs as Modulators of pre-mRNA Splicing 540
Chapter 31. Selective RNA Cleavage by Isolated RNase L Acti- vated with 2–5A Antisense Chimeric Oligonucleotides 556
Author Index 569
Subject Index 601