Redox Cell Biology and Genetics, Part A (eBook)

Cover 1
Table of Contents 6
Contributors to Volume 352 10
Preface 16
Volumes in Series 18
Section I: Cellular Responses 38
Chapter 1. Measurement of Absolute Oxygen Levels in Cells and Tissues Using Oxygen Sensors and 2-Nitroimidazole EF5 40
Chapter 2. Detection of Oxygen-Sensing Properties of Mitochondria 68
Chapter 3. Direct Detection of Singlet Oxygen via Its Phosphorescence from Cellular and Fungal Cultures 78
Chapter 4. Molecular Analysis of Mitogen-Activated Protein Kinase Signaling Pathways Induced by Reactive Oxygen Intermediates 90
Chapter 5. Detection of Intracellular Reactive Oxygen Species in Cultured Cells Using Fluorescent Probes 99
Chapter 6. Flow Cytometric Determination of Cytoplasmic Oxidants and Mitochondrial Membrane Potential in Neuronal Cells 108
Chapter 7. Flow Cytometric Determination of Lipid Peroxidation Using Fluoresceinated Phosphoethanolamine 117
Chapter 8. Determination of Intracellular Reactive Oxygen Species as Function of Cell Density 128
Chapter 9. Identification of Redox-Active Proteins on Cell Surface 138
Chapter 10. Probing Redox Activity of Human Breast Cells by Scanning Electrochemical Microscopy 149
Chapter 11. Determining Influence of Oxidants on Nuclear Transport Using Digitonin-Permeabilized Cell Assay 160
Chapter 12. Functional Imaging of Mitochondrial Redox State 172
Chapter 13. Hydrogen Peroxide-Induced Apoptosis: Oxidative or Reductive Stress? 187
Chapter 14. Peroxidation of Phosphatidylserine in Mechanisms of Apoptotic Signaling 196
Chapter 15. Quantitative High Throughput Endothelial Cell Migration and Invasion Assay System 212
Chapter 16. In Vitro Model of Oxidative Stress in Cortical Neurons 220
Chapter 17. Glutamate-Induced c-Src Activation in Neuronal Cells 228
Chapter 18. Measurement of Inflammatory Properties of Fatty Acids in Human Endothelial Cells 235
Chapter 19. Redox Control of Tissue Factor Expression in Smooth Muscle Cells and Other Vascular Cells 257
Chapter 20. Redox Processes Regulate Intestinal Lamina Propria T Lymphocytes 269
Chapter 21. Linker for Activation of T Cells: Sensing Redox Imbalance 285
Chapter 22. Generation of Prooxidant Conditions in Intact Cells to Induce Modifications of Cell Cycle Regulatory Proteins 295
Chapter 23. Analysis of Transmembrane Redox Reactions: Interaction of Intra- and Extracellular Ascorbate Species 305
CH4Chapter 24. Regulation of Endothelial Cell Proliferation by Nitric Oxide 317
Chapter 25. Fluorescent Imaging of Mitochondrial Nitric Oxide in Living Cells 333
Section II: Tissues and Organs 342
Chapter 26. Detection of Reactive Oxygen and Nitrogen Species in Tissues Using Redox-Sensitive Fluorescent Probes 344
Chapter 27. Simultaneous Detection of Tocopherols and Tocotrienols in Biological Samples Using HPLCCoulometric Electrode Array 363
Chapter 28. In Vivo Measurement of Oxidative Stress Status in Human Skin 370
Chapter 29. Localization of Oxidation-Specific Epitopes in Tissue 377
Chapter 30. Quantitation of S-Nitrosothiols in Cells and Biological Fluids 384
Chapter 31. Peroxisomal Fatty Acid Oxidation and Cellular Redox 398
Chapter 32. Ultrastructural Localization and Relative Quantification of 4-Hydroxynonenal-Modified Proteins in Tissues and Cell Compartments 410
Chapter 33. Ultrastructural Localization of Light-Induced Lipid Peroxides 415
Chapter 34. A Survival Model for Study of Myocardial Angiogenesis 428
Chapter 35. Determination of Angiogenesis-Regulating Properties of NO 444
Chapter 36. Hemangioma Model for in Vivo Angiogenesis: Inducible Oxidative Stress and MCP-1Expression in EOMA Cells 459
Chapter 37. Redox Aspects of Vascular Response to Injury 469
Chapter 38. Involvement of Superoxide in Pathogenic Action of Mutations That Cause Alzheimer’s Disease 492
Chapter 39. Three-Dimensional Redox Imaging of Frozen- Quenched Brain and Other Organs 512
Chapter 40. In Vivo Fluorescent Imaging of NADH Redox State in Brain 519
Chapter 41. Nitroxyl Probes for Brain Research and Their Application to Brain Imaging 531
Chapter 42. Analytical Implications of Iron Dithiocarbamates for Measurement of Nitric Oxide 543
Author Index 560
Subject Index 598