Laser Capture in Microscopy and Microdissection (eBook)

Cover 1
Table of Contents 6
Contributors to Volume 356 10
Preface 14
Volumes in Series 16
Section I: Basic Principles 38
Chapter 1. Comparison of Current Equipment 40
Chapter 2. Laser Capture Microdissection and Its Applications in Genomics and Proteomics 49
Chapter 3. Going in Vivo with Laser Microdissection 62
Chapter 4. Use of Laser Capture Microdissection to Selectively Obtain Distinct Populations of Cells for Proteomic Analysis 70
Chapter 5. Optimized Tissue Processing and Staining for Laser Capture Microdissection and Nucleic Acid Retrieval 86
Chapter 6. Fluorescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections 100
Chapter 7. Immunoblotting of Single Cell Types Isolated from Frozen Sections by Laser Microdissection 107
Chapter 8. Noncontact Laser Catapulting: A Basic Procedure for Functional Genomics and Proteomics 117
Chapter 9. Internal Standards for Laser Microdissection 136
Chapter 10. Methacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded Tissues 151
Section II: Specialized Uses 164
Chapter 11. Use of Laser Capture Microdissection for Clonal Analysis 166
Chapter 12. Laser Capture Microdissection in Carcinoma Analysis 173
Chapter 13. Laser Capture Microdissection to Assess Development 181
Chapter 14. Application of Laser Capture Microdissection to Proteomics 193
Chapter 15. Laser Capture Microdissection of Mouse Intestine: Characterizingm mRNA and Protein Expression, and Profiling Intermediary Metabolism in Specified Cell Populations 203
Chapter 16. Laser Capture Microdissection in Pathology 232
Chapter 17. Use of Laser Capture Microscopy in the Analysis of Mouse Models of Human Diseases 243
Chapter 18. Use of Laser Microdissection in Complex Tissue 252
Chapter 19. Assessment of Clonal Relationships in Malignant Lymphomas 260
Chapter 20. Comparison of Normal and Tumor Cells by Laser Capture Microdissection 276
Chapter 21. Analysis of Folliculostellate Cells by Laser Capture Microdissection and Reverse Transcription–Polymerase Chain Reaction (LCMRT/ PCR) 284
Section III: Genetic Applications 293
Chapter 22. Analysis of Gene Expression 295
Chapter 23. Analysis of Specific Gene Expression 307
Chapter 24. Gene Discovery with Laser Capture Microscopy 318
Chapter 25. DNA Fingerprinting from Cells Captured by Laser Microdissection 325
Chapter 26. Single Cell PCR in Laser Capture Microscopy 331
Chapter 27. Assessment of Genetic Heterogeneity in Tumors Using Laser Capture Microdissection 338
Chapter 28. Gene Mutations: Analysis in Proliferative Prostatic Diseases Using Laser Capture Microdissection 345
Chapter 29. Use of Laser Capture Microdissection-Generated Targets for Hybridization of High-Density Oligonucleotide Arrays 359
Chapter 30. Single Cell Gene Mutation Analysis Using Laser- Assisted Microdissection of Tissue Sections 370
Chapter 31. Methylation in Gene Promoters: Assessment after Laser Capture Microdissection 379
Author Index 390
Subject Index 412