Ribonucleases, Part A: Functional Roles and Mechanisms of Action (eBook)

Front Cover 1
Ribonucleases 4
Copyright Page 5
Table of Contents 6
Contributors to Volume 341 10
Preface 16
Volume in Series 18
Section I: Ribonuclease Classification and Review 38
Chapter 1. A Natural Classification of Ribonucleases 40
Chapter 2. The Ribonuclease T1 Family 65
Chapter 3. Ribonuclease T2 79
Chapter 4. The Ribonuclease P Family 93
Section II: Ribonuclease Assays 116
Chapter 5. Fast, Facile, Hypersensitive Assays for Ribonucleolytic Activity 118
Chapter 6. Activity Staining for Detection of Ribonucleases Using Dried Agarose Film Overlay Method after Isoelectric Focusing 131
Chapter 7. Gel Renaturation Assay for Ribonucleases 150
Chapter 8. Analysis of Ribonucleases Following Gel Electrophoresis 163
Chapter 9. Ribonuclease Assays Utilizing Toluidine Blue Indicator Plates, Methylene Blue, or Fluorescence Correlation Spectroscopy 179
Chapter 10. Ribonuclease Activities of Trypanosome RNA Editing Complex Directed to Cleave Specifically at a Chosen Site 191
Chapter 11. Ribonuclease YI*, RNA Structure Studies, and Variable Single-Strand Specificities of RNases 212
Section III: Secreted Ribonucleases 224
Chapter 12. Bovine Pancreatic Ribonuclease A: Oxidative and Conformational Folding Studies 226
Chapter 13. Purification of Engineered Human Pancreatic Ribonuclease 258
Chapter 14. Degradation of Double-Stranded RNA by Mammalian Pancreatic-Type Ribonucleases 271
Chapter 15. Seminal Ribonuclease: Preparation of Natural and Recombinant Enzyme, Quaternary Isoforms, Isoenzymes, Monomeric Forms Assay for Selective Cytotoxicity of the Enzyme
Chapter 16. Angiogenin 300
Chapter 17. Eosinophil-Derived Neurotoxin 310
Chapter 18. Eosinophil Cationic Protein 324
Chapter 19. Deciphering the Mechanism of RNase T1 342
Chapter 20. Mitogillin and Related Fungal Ribotoxins 361
Chapter 21. RNase U2 and ?-Sarcin: A Study of Relationships 372
Chapter 22. Secretory Acid Ribonucleases from Tomato, Lycopersicon esculentum Mill. 388
Chapter 23. Leczyme 405
Section IV: Ribonucleases H 412
Chapter 24. Prokaryotic Type 2 RNases H 414
Chapter 25. RNase H1 of Saccharomyces cerevisiae: Methods and Nomenclature 432
Chapter 26. Ribonucleases H of the Budding Yeast, Saccharomyces cerevisiae 451
Chapter 27. Human RNases H 467
Chapter 28. Assays for Retroviral RNase H 477
Section V: Synthetic Ribonucleases 490
Chapter 29. Sequence-Selective Artificial Ribonucleases 492
Chapter 30. RNA Cleavage by 1,4-Diazabicyclo[2.2.2]- octane–Imidazole Conjugates 505
Chapter 31. Preparation and Use of ZFY-6 Zinc Finger Ribonuclease 527
Section VI: Ribonucleolytic Nucleic Acids 538
Chapter 32. RNA Cleavage by the 10-23 DNA Enzyme 540
Chapter 33. Leadzyme 555
Chapter 34. Hammerhead Ribozyme Structure and Function in Plant RNA Replication 577
Chapter 35. Kinetic Analysis of Bimolecular Hepatitis delta Ribozyme 590
Chapter 36. Catalytic and Structural Assays for the Hairpin Ribozyme 603
Chapter 37. Intracellular Applications of Ribozymes 618
Section VII: Ribonuclease Inhibitors 634
Chapter 38. Barnase–Barstar Interaction 636
Chapter 39. Cytoplasmic Ribonuclease Inhibitor 648
Chapter 40. Small Molecule Inhibitors of RNase A and Related Enzymes 666
Chapter 41. Ribonuclease-Resistant RNA Controls and Standards 685
Section VIII: Nonenzymatic Cleavage of RNA 692
Chapter 42. Nonenzymatic Cleavage of Oligoribonucleotides 694
Author Index 714
Subject Index 754