The Genus Yersinia: (eBook)

Contributors 6
Contents 18
Part I - Genomics 26
Comparative Genome Analyses of the Pathogenic Yersiniae Based on the Genome Sequence of Yersinia enterocolitica Strain 8081 27
Pestoides F, an Atypical Yersinia pestis Strain from the Former Soviet Union 42
Variability of the Protein Sequences of LcrV Between Epidemic and Atypical Rhamnose- Positive Strains of Yersinia pestis 48
A New Asset for Pathogen Informatics – the Enteropathogen Resource Integration Center ( ERIC), an NIAID Bioinformatics Resource Center for Biodefense and Emerging/ Re- emerging Infectious Disease 53
Part II – Structure and Metabolism 68
My Life with Yersinia 69
Structure and Assembly of Yersinia pestis F1 Antigen 99
Relationship of the Lipopolysaccharide Structure of Yersinia pestis to Resistance to Antimicrobial Factors 113
Characterization of Six Novel Chaperone/Usher Systems in Yersinia pestis 122
Polyamines in Bacteria: Pleiotropic Effects yet Specific Mechanisms 131
Intermediary Metabolism, Na+, the Low Calcium- Response, and Acute Disease 141
Part III – Regulatory Mechanisms 155
Differential Gene Regulation in Yersinia pestis versus Yersinia pseudotuberculosis: Effects of Hypoxia and Potential Role of a Plasmid Regulator 156
Two-Component System Regulon Plasticity in Bacteria: A Concept Emerging from Phenotypic Analysis of Yersinia pseudotuberculosis Response Regulator Mutants 170
Regulatory Elements Implicated in the Environmental Control of Invasin Expression in Enteropathogenic Yersinia 181
Regulation of the Phage-Shock-Protein Stress Response in Yersinia enterocolitica 192
Functional Quorum Sensing Systems Affect Biofilm Formation and Protein Expression in Yersinia pestis 203
Analysis of Yersinia pestis Gene Expression in the Flea Vector 217
Regulation of Biofilm Formation in Yersinia pestis 226
Environmental Stimuli Affecting Expression of the Ysa Type Three Secretion Locus 236
Polynucleotide Phosphorylase and the T3SS 242
Roles of YopN, LcrG and LcrV in Controlling Yops Secretion by Yersinia pestis 250
Identification of TyeA Residues Required to Interact with YopN and to Regulate Yop Secretion 260
Part IV – Pathogenesis and Host Interactions 271
The Insect Toxin Complex of Yersinia 272
Twin Arginine Translocation in Yersinia 283
Using Every Trick in the Book: The Pla Surface Protease of Yersinia pestis 293
Invasion and Dissemination of Yersinia enterocolitica in the Mouse Infection Model 304
The Ysa Type 3 Secretion System of Yersinia enterocolitica Biovar 1B 311
A Rationale for Repression and/or Loss of Motility by Pathogenic Yersinia in the Mammalian Host 323
Disparity Between Yersinia pestis and Yersinia enterocolitica O: 8 in YopJ/ YopP- Dependent Functions 337
Part V – Molecular Epidemiology and Detection 346
3 IS-RFLP: A Powerful Tool for Geographical Clustering of Global Isolates of Yersinia pestis 347
Analysis of the Three Yersinia pestis CRISPR Loci Provides New Tools for Phylogenetic Studies and Possibly for the Investigation of Ancient DNA 352
Enrichment of Yersinia pestis from Blood Cultures Enables Rapid Antimicrobial Susceptibility Determination by Flow Cytometry 364
Development and Evaluation of a Single Tube Nested PCR Based Approach ( STNPCR) for the Diagnosis of Plague 376
Part VI – Vaccine and Antimicrobial Therapy Development 385
Therapeutic Potential of Yersinia Anti-Inflammatory Components 386
High Throughput Screening for Small-Molecule Inhibitors of Type III Secretion in Yersinia pestis 392
Cell-Mediated Defense Against Yersinia pestis Infection 401
Oral Vaccination with Different Antigens from Yersinia pestis KIM Delivered by Live Attenuated Salmonella Typhimurium Elicits a Protective Immune Response Against Plague 412
Yersinia pestis YadC: A Novel Vaccine Candidate Against Plague 425
Protective Immunity Against Plague 440
Index 450