Making and Using Antibodies

Written for professionals in the fields of biomedical research, this book uses a cookbook approach to presenting methods for the production, characterization, and use of antibodies. It covers basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry.

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Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols.

Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers-
Commercially available adjuvants designed for the production of antisera in the research setting

Methods for the production, purification, and characterization of antibodies

Practical guidance to researchers needing to modify antibodies

Basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry

Methods for applying immunolabeling to the realm of electron microscopy (EM)

New antibody sources and new ways to use antobodies

Making and Using Antibodies: A Practical Handbook also offers invaluable insight into future directions, challenges, and opportunities both in research and industrial applications.