Molecular Endocrinology

Determining the Affinity of Hormone?Receptor Interaction.- Determination of Serum Estradiol Levels by Radiometric and Chemiluminescent Techniques.- Identification of Natural Human Glucocorticoid Receptor (hGR) Mutations or Polymorphisms and Their Functional Consequences at the Hormone–Receptor Interaction Level.- Monitoring Insulin-Stimulated Production of Signaling Lipids at the Plasma Membrane.- Gene Expression Profiling in the Aging Ovary.- Genomics Analysis: Endometrium.- Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization.- Adenoviral Gene Transfer into Isolated Pancreatic Islets.- Basic Molecular Techniques for the Detection of Single Nucleotide Polymorphisms: Genome-Wide Applications in Search for Endocrine Tumor Related Genes.- Methylated DNA Immunoprecipitation and Microarray-Based Analysis: Detection of DNA Methylation in Breast Cancer Cell Lines.- Use of Reporter Genes to Study the Activity of Promoters in Ovarian Granulosa Cells.- Use of Reporter Genes to Study Promoters of the Androgen Receptor.- Isolation of Proteins Associated with the DNA-Bound Estrogen Receptor ?.- Chromosome-Wide Analysis of Protein Binding and Modifications.- Genome-Wide Analysis for Protein?DNA Interaction: ChIP-Chip.- Detection of ER?-SRC-1 Interactions Using Bioluminescent Resonance Energy Transfer.- Detection of Proteins Sumoylated In Vivo and In Vitro.- Identification of Alternative Transcripts Using Rapid Amplification of cDNA Ends (RACE).- Use of Laser Capture Microdissection in Studying Hormone-Dependent Diseases: Endometriosis.- Reporter Mice for the Study of Intracellular Receptor Activity.- Real-Time Non-invasive Imaging of ES Cell-Derived Insulin Producing Cells.- Transgenic Mouse Technology: Principles and Methods.- Breast Tumor-InitiatingCells Isolated from Patient Core Biopsies for Study of Hormone Action.- Markers of Oxidative Stress and Sperm Chromatin Integrity.- Planning and Executing a Genome Wide Association Study (GWAS).