A Laboratory Guide for Cellular and Molecular Plant Biology
Springer Basel (Verlag)
978-3-7643-2542-8 (ISBN)
Foreword.- 1 Cellular Techniques - General Introduction.- 1.1 Isolation and Culture of Protoplasts.- 1.2 Mutagenic Treatments on Isolated Cells.- 1.3 Somatic Hybridization.- 1.4 Dilution Series as a Tool to Improve Medium Composition.- 1.5 Plating Efficiency Evaluation in a Peroxidase Assay.- 1.6 Production of Haploid Plants.- 1.7 Isolation of Viable Microspores and Immature Pollen Grains from Cereal Inflorescences.- 1.8 Isolation of Viable Sperm Cells from Corn (Zea mays) Pollen Grains.- 1.9 Embryo Rescue in Nicotiana plumbaginifolia.- 1.10 Use of Iodide Ions for Chemical Reduction of the Oxidative Agent H2O2 and Hypochlorites after Application as Decontaminating Agents for Plant Tissues.- 2 Transformation Techniques - General Introduction.- 2.1 Agrobacterium Transformation of Various Arabidopsis Expiants.- 2.2 Direct Gene Transfer.- 3 Extraction Techniques - General Introduction.- 3.1 Isolation of DNA and RNA from Arabidopsis thaliana.- 3.2 Total DNA Extraction - Alternative Protocols.- 3.3 Characterization of Mitochondrial-DNA from Minute Quantities of Plant Material.- 3.4 Generation of Large Amounts of cDNA by Polymerase Chain Reaction from Small Amounts of Total RNA.- 3.5 Isolation of Nuclei from Plant Tissues.- 3.6 Extraction of Amino Acids from Plant Samples and Their Analysis Using Ion-Exchange Chromatography.- 3.7 Electroelution of Proteins from Plant Tissues.- 3.8 Extraction, Purification and Analysis of Endogenous Indoleacetic Acid and Abscisic Acid.- 4 Aspects of Structural and Functional Analysis of Genomes and Genes - General Introduction.- 4.1 Southern Blot Analysis of Transgenic Nicotiana sp.- 4.2 Northern Blot Analysis of ADH (Alcohol Dehydrogenase) Mutants in Arabidopsis.- 4.3 Western Blot Detection of Proteins Synthesized Transiently inTransfected Plant Protoplasts.- 4.4 Cloning Nuclear Single Copy Sequences for RFLP Analysis.- 4.5 Run-on Transcription in Isolated Plant Nuclei.- 4.6 Preparation of Nuclear Extracts, Gel-Retardation Assay and DNAase I Footprinting.- 4.7 Pulsed-Field Gel Electrophoresis of Plant DNA.- 4.8 Assessing Methylation of Inserted DNA by Restriction with Isoschizomeric Enzymes and Inducing Demethylation with 5-Azacytidine.- 5 Cytological Techniques - General Introduction.- 5.1 Karyotyping with Protoplast Procedures.- 5.2 In situ Hybridization.- 5.3 How Cytometry of Nuclei for Ploidy and Cell Cycle Analysis.- 5.4 Control of Cell Cycle Progression.- 5.5 Induction and Isolation of Micronuclei and Microprotoplasts.- 6 Appendices.- 6.1 Culture Media and Basic Stock Solution.- 6.4 Extraction and Purification of IAA and ABA.- 7 Subject Index.
Erscheint lt. Verlag | 1.11.1991 |
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Reihe/Serie | Biomethods |
Zusatzinfo | 388 p. |
Verlagsort | Basel |
Sprache | englisch |
Maße | 170 x 244 mm |
Gewicht | 894 g |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Sozialwissenschaften | |
Schlagworte | Arabidopsis thaliana • Biotechnologie • Botanik • DNA • Embryo • genes • gene transfer • Hardcover, Softcover / Naturwissenschaften allgemein • HC/Naturwissenschaften allgemein • Hybridization • IAA • Molekularbiologie • Pflanzenzelle • Southern Blot • transcription • Vererbung |
ISBN-10 | 3-7643-2542-9 / 3764325429 |
ISBN-13 | 978-3-7643-2542-8 / 9783764325428 |
Zustand | Neuware |
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