Steroid-Cell Interactions (eBook)

Front Cover 
1 
Steroid - Cell Interactions 
4 
Copyright Page 
5 
Table of Contents 
8 
Preface 6
Introduction 17
Chapter 1. Physico-Chemical Considerations of Steroid-Receptor Interactions 
20 
STEROID STRUCTURE 20
PROTEIN STRUCTURE 24
NUCLEIC ACIDS 25
CHEMICAL BONDS 25
EQUILIBRIUM CONSTANT AND FREE ENERGY 28
REFERENCES 35
Chapter 2. Methods Used to Study Steroid-Tissue 
36 
CELL FRACTIONATION 36
CELL TYPE 38
AUTORADIOGRAPHY 39
DISTINCTION BETWEEN HIGH AND LOW AFFINITY BINDING SITES 40
REFERENCES 42
Chapter 3. The Importance of the Metabolism of Steroid Hormones Relative to their Binding and Mechanism of Action 
44 
THE ABSENCE OF THE METABOLISM OF STEROID HORMONES DURING THEIR UPTAKE AND RETENTION IN MANY TARGET CELLS 
44 
THE METABOLISM OF TESTOSTERONE IN ANDROGEN-DEPENDENT TISSUES 46
THE METABOLISM OF PROGESTERONE IN CERTAIN TARGET TISSUE 54
REFERENCES 57
Chapter 4. 
60 
BLOOD 60
THE DISTRIBUTION OF NADPH-DEPENDENT 5a-REDUCTASE AND RECEPTORS FOR 5a-DIHYDROTESTOSTERONE IN ANDROGEN-SENSITIVE TISSUES AND CELLS 
69 
THE UPTAKE OF TESTOSTERONE INTO ANDROGEN-SENSITIVE ANDANDROGEN-DEPENDENT TISSUES 73
THE SPECIFIC, HIGH AFFINITY BINDING OF 5a-DIHYDR0-TESTOSTERONE IN THE RAT VENTRAL PROSTATE GLAND 75
BINDING OF 5a-DIHYDROTESTOSTERONE IN ACCESSORY SEXUAL GLANDS OTHER THAN RAT VENTRAL PROSTATE GLAND 
102 
SEARCHES FOR RECEPTORS IN OTHER ANDROGEN-SENSITIVE SYSTEMS 
104 
BINDING OF ANDROGENS IN OTHER TISSUES AND CELLS 110
GENERAL SUMMARY 113
REFERENCES 114
Chapter 5. Glucocorticoids 
121 
BLOOD 121
SPECIFIC GLUCOCORTICOID RECEPTOR PROTEINS 128
LIVER 129
HTC CELLS 136
HeLa CELLS 152
LYMPHOCYTES 156
LYMPHOID TUMOURS 165
FIBROBLASTS 168
OTHER TISSUES 173
GENERAL SUMMARY 174
REFERENCES 176
Chapter 6. Mineralocorticoids 181
BLOOD 181
THE SEARCH FOR SPECIFIC ALDOSTERONE-BINDING PROTEINS 184
RAT KIDNEY 187
TOAD BLADDER 200
OTHER TISSUES 203
GENERAL SUMMARY 205
REFERENCES 206
Chapter 7. Oestrogens 209
BLOOD 209
ENTRY OF OESTROGEN INTO CELLS 213
UTERUS 219
VAGINA 258
PITUITARY GLAND 259
BRAIN 260
OVARY 266
KIDNEY 266
PROSTATE AND SEMINAL VESICLE 267
OVIDUCT 267
MAMMARY GLAND 268
TUMOURS 268
OTHER TISSUES 274
REFERENCES 274
Chapter 8. Progesterone 
282 
BLOOD 282
OVIDUCT 287
UTERUS 296
OTHER TISSUES 303
REFERENCES 304
Chapter 9. Relationship of Binding to Biological Activity 
307 
INTRODUCTION 307
EVIDENCE THAT THE STEROID-RECEPTOR INTERACTION IS RELATED TO THE BIOLOGICAL ACTIVITY OF THE STEROID 
307 
PHYSIOLOGICAL ASPECTS OF STEROID-RECEPTOR INTERACTION 335
THE MOLECULAR ASPECTS OF STEROID-RECEPTOR INTERACTION 350
REFERENCES 385
Chapter 10. Clinical and Immunological Aspects of Steroid-Binding 
398 
TERMINOLOGY 398
PLASMA PROTEINS 399
TISSUE PROTEINS 401
STEROID ANTIBODIES 402
REFERENCES 408
Appendix 1. Chemical and Trivial Names of Steroids 410
Appendix 2. Structure of synthetic Compound 415
Appendix 3. An Up-to-Date Summary of Current Literature 
417 
Index 450