Protides of the Biological Fluids (eBook)

Front Cover 
1 
Protides of the Biological Fluids 4
Copyright 5
Table of Contents 6
Preface 14
Acknowledgements 16
ACADEMIC LECTURE Analyses Immunochimiques des Tissus 18
SECTION A: CONFORMATION AND STRUCTURE OF PROTEINS 36
A1. Protein Models 38
Polypeptides as Models for the Tertiary Structure of Proteins 38
ß-Lactoglobulin as a Model of Subunit Enzymes 50
Preferential Binding of Solvent Components to Protein in Mixed Water–Organic Solvent Systems 58
Some Aspects of Protein Models and Biological Function 68
Uses and Limitations of Various Types of Atomic Models in Molecular Representation 82
Chemical, Morphological and Immunologic Properties of Recombined Red Cell Membranes 88
A2. Conformation and Dielectric Behaviour 98
Messungen der Dielektrizitätskonstanten von Hämoglobinstrukturänderungen in Wässriger Lösung mit einem Mikrowelleninterfer... 98
R. F. Measurements of the Dielectric Properties of Strongly Conducting Liquids 104
Size and Shape Determination of Globular Proteins I. Bridge and Cell Problems in the Dielectric Measurement of Protein Solut... 112
Size and Shape Determination of Globular Proteins. II. Dielectric Relaxation and Viscosity Determination of Globular Proteins 118
The Dipole Moment in Sperm Whale Myoglobin 126
A3. Structure by Optical Methods 132
Application de la Diffusion de la Lumière à l'Étude de quelques Macromolécules Biologiques 132
Optical Rotation Studies on Globular Serum Proteins and Isolated Polypeptide Chains of Immunoglobulins 144
On the ß-Conformation in Eye-lens Proteins 154
Messung der Optischen Rotationsdispersion von Nativer und Modifizierter Lactatdehydrogenase sowie des Enzym–Coenzym-Kompl... 160
Circular Dichroism of the ß-Lactoglobulins between 190 and 320 nm 166
A4. Structure by Other Methods 172
Elektronenmikroskopische Untersuchung zur Struktur von Fibrinfibrillen 172
Study of the Alkaline Bohr Effect of Hemoglobin by means of Differential Hydrogen Ion Titration Curves 178
Kinetic Studies of Antibody–Hapten Reactions with the Temperature-jump Relaxation Technique 184
A5. Structure of Lysozyme 190
The Structure and Function of Lysozyme 190
Relations entre Structure et Activité chez quelques Lysozymes de Blancs d'Œeufs d'Oiseaux et d'Origine Humaine 198
Constantes Apparentes d'Affinité de Lysozymes d'Origines Diverses pour Micrococcus lysodeikticus 208
Immunochemical Analyses of Human Lysozyme in Monocytic Dyscrasias 214
A6. Structure of Immunoglobulins 222
Structure and Cellular Localization of Secretory IgA 222
The Secretory Immunologic System 238
Les Fragments de Protéolyse Enzymatique des IgM Globulines Humaines 244
Rapports des Équivalents Structuraux de la Gamma G et de la Gamma A vis-à-vis de Certaines Activités Biologiques 250
A7. Structure of Various Proteins 258
Nature and Functions of Mucins and Blood Group Substances 258
Dissociation of Human a2-Macroglobulin at Acid and Alkaline pH 264
Structure Quaternaire des Alpha-2-macroglobulines Humaines Normales 272
Untersuchungen zur Lokalisation der Insertionsstellen Insulinbindender Antikörper am Insulinmolekül 282
SECTION B: PROTEINS OF BODILY SECRETIONS 290
B1. General Papers 292
Studies of One Specialized Local Antibody System 292
Studies on Immunoglobulins of Secretions in Dogs and Mice 300
The Fundamental Structure of the Glycoproteins in Mucous Secretions 304
The Compositional Pattern of the Carbohydrates of Glycoproteins of Epithelial Secretions 310
The Composition of Mucus with Special Reference to its Rheological Properties 316
B2. Nasal Secretions 324
Factors Influencing the Production of Secretory IgA Antibody in Nasal Secretions 324
The Biologic Significance of IgA in the Mucous Secretions: Response to Oral Polio Vaccine in IgA-deficient Subjects 332
Localization of External Secretory IgA Piece by Immunofluorescence in Tissues from Patients with Agammaglobulinemia 342
Local Synthesis of Immunoglobulin G in the Upper Respiratory Tract 348
Protein and Lipid Pattern in Serous Otitis Media 354
B3. Bronchial Secretions 360
Biochemical Exploration of Bronchial Hypersecretions 360
Etude des Glycopeptides du Mucus Fibrillaire de la Sécrétion Bronchique 378
Secretions of Proteins into the Sputum in Chronic Bronchitis 388
B4. Gastro-Intestinal Secretions–Saliva 396
Mucous in Normal Human Submaxillary Saliva 396
Der Nachweis von M-Komponenten der Immunglobuline im Speichel von Patienten mit Paraproteinämien 402
The Relation between the Histology of the Gastric Mucosa, Secretion of Intrinsic Factor, Autoimmunity and the Absorption o... 410
The Vitamin B12, Transport Proteins in Body Fluids and Leucocytes 418
Etude de Deux Transporteurs de Vitamine B12, Isolés de la Muqueuse Gastrique de Porc 426
Autoimmunization to Intrinsic Factor and Immunization to Oral Hog Intrinsic Factor in Man–Some Characteristic Features 440
Rôle des Glycoprotéines Sulfatées dans les Méchanismes de Protection de la Muqueuse Gastrique 446
Antibody Content of Anacid Gastric Juices: The occurrence of E. coli Antibodies and Poliovirus Neutralizing Antibodies 452
Über die Isolierung und Immunchemische Charakterisierung der Antigenen Magensaftesterase VI A 458
Isolement et Caractérisation de Chondro.tine-4-Sulfate dans la Sécrétion Gastrique Fundique chez le Chien 468
Coproantibody and Defense Mechanisms of the Intestinal Mucosal Surface 476
Immunoglobulin Production and Secretion into the Intestinal Tract of Primates under the Influence of Some Antigenic Stimuli 486
Immunoglobulin Synthesis by Tissues of the Gastro-intestinal and Respiratory Tracts 496
Increased Serum IgA in Intestinal Disease 502
Source of IgA in Jejunal Secretions 508
Protein Synthesis by the Human Exocrine Pancreas 516
Présence d'une Protéine d'Origine Tissulaire, l's2H dans le Sérum de Sujets atteints d'Affections Malignes 528
First Meconium in the Detection of Fibrocystic Disease of the Pancreas 534
B5. Genital Secretions 542
Muramidase (Lysozyme) in Cervical Secretions 542
Enzymes and Proteins in Uterine Mucus 552
A Seminal Fluid Component used for Desensibilization of Women with Unexplained Infertility 558
The Antigens of Human Seminal Plasma (with Special Reference to Lactoferrin as a Spermatozoa-coating Antigen) 566
Purification of the 3.72 S Protein of the Human Seminal Fluid by Fractionation on Sephadex G 200 and Preparative Agar Gel Electrophoresis 570
B6. Urinary Proteins 576
"Tissue" Antigens and Enzymes in Human Urine 576
Caractérisation et Séparation du Pepsinogène Urinair: Corrélations avec la Sécrétion Gastrique 590
Polymorphisme Immunologique des Molécules de .G Excrétées au cours de Protéinuries 602
Les Facteurs Gm, Inv et ISf des Urines Normales 610
Nachweis Elektrophoretisch Aufgetrennter Harnkolloide durch Kaliumpermanganat 614
The Level of Plasma Proteins in Normal Human Urine 620
Les Mucopolysaccharides Urinaires dans l'Epilepsie Myoclonique Progressive Familiale (types Lafora et Unverricht–Lundborg) 628
B7. Milk Proteins 636
Ekkrinosiderophilin of Human Milk 636
The Role of Lactoferrin in Ion Absorption 644
New Data on Lactoferrin, the Iron-binding Protein of Secretions 650
The Origin of Immunoglobulin in Ruminant Milk 656
Isolement et Caractérisation de l' Auto-antigène, Responsable de la Formation d'Auto-anticorps chez les Malades Atteints de... 664
Comparison of Rabbit Immunoglobulins–Colostral IgA 676
Preferential Adsorption of a Single Bovine IgG Type by Isolated Epithelial Cells of the Mammary Gland 680
B8. Extra-cellular Fluid Proteins 686
Les Immunoglobulines Seriques IgG, IgA et IgM dans les Liquides Ascitiques de Nature Différente 686
Extravascular Plasma Albumin in the Kidney and Its Possible Role in Urine Formation 690
Les Antigènes du Liquide Amniotique Humain 696
The Transcapillary Passage of Different Proteins in the Operative Wound 702
SECTION C. TECHNIQUES 710
C1. Electro-focusing 712
Isoelectric Focusing of an Estradiol Bindings ß-Globulin Present in Human Serum 712
Electro-focusing Studies in the Protein Hormones–Human Chorionic Gonadotrophin, Human Luteinizing Hormone and Pregnant Ma... 718
The Subunits of Low- and High-density Lipoproteins 724
C2. Determination of Fractions 732
The Stokes Radius of Macromolecules Determined from Electrophoresis in Agarose 732
Lipoprotein Electrophoresis on Gelatinized Cellulose Acetate 740
Free Ammo Acids of the Blood Plasma in Cases of Megaloblastic Anaemia 748
The Separation and Quantitative Analysis of Gamma-aminobutyric Acid (Gaba) by One-dimensional Paper Chromatography 752
Isolation of Components of Fragmented Normal Human IgG Immunoglobulin 758
A Longitudinal Study of the Serum Ceruloplasmin, a2-Macroglobulin, Transferrin and Immunoglobulins IgA, IgG and IgM in Chi... 764
The Turnover in Humans of Polymerized Human Serum Albumin and of Fractions of Human Serum Albumin with Different N–F Trans... 772
Variations in Protein Synthesis Rate Demonstrated with the Fluorescent Antibody Technique 780
INDEX 786