Immunoassay of Hormones, Volume 14 (eBook)

Sir Gordon Ethelbert Ward Wolstenholme OBE FRCP was a British medical doctor, and the founding director of the Ciba Foundation. He was born in Sheffield, the son of a mechanical engineer, and educated at Repton School and Corpus Christi, Cambridge.

Immunoassay of Hormones 5
Contents 10
Chairman’s Opening Remarks 15
Basic immunological considerations 20
Questions 28
Some aspects of immunochemical methods for the characterization of protein hormones 34
Questions 49
General Discussion 54
Experiences with the haemagglutination method of human growth hormone assay 62
Questions 78
Immunochemical studies on human growth hurmone: a consideration of the human growth hormone-anti-human growth hormone system and its application to the assay of growth hormone 80
Questions 127
Immunological studies with human growth hormone 130
Questions 140
Immunoassay of serum growth hormone in diabetes mellitus 142
Questions 157
Discussion on Growth Hormone 161
Immunological studies of insulin 171
Questions 191
Endogenous and pancreatic insulins 195
Questions 203
Immunoassay of plasma insulin 208
Questions 229
Discussion on Insulin 232
Demonstration of the hormonal status of endogenous glucagon 240
Discussion on Glucagon 249
Some facets of thyrotropin immunoassay 253
Some studies with antiserum to thyrotropin 266
Immunobiological studies of thyrotropin 276
Discussion on Thyrotropin 292
Studies with antisera to corticotropin (ACTH) 302
Discussion on Corticotropin 316
Determination of gonadotropins in urine by a haemagglutination inhibition reaction 325
Questions 336
Immunological investigations of human gonadotropins 339
Questions 353
Discussion on Gonadotropins 354
Short Communication: Determination of human chorionic gonadotropin in biological fluids by complement fixation 358
Discussion on Gonadotropins ( contd.) 363
Immunological studies with pituitary lactogenic hormone (prolactin) 367
Discussion on Prolactin 394
Panel Discussion: Intracellular detection of hormones by immuno- chemical means 407
Growth hormone 407
Other pituitary hormones 410
Other protein hormones 413
Discussion 414
General Discussion 427