Bioassay of Anterior Pituitary and Adrenocortical Hormones, Volume 5 (eBook)

Sir Gordon Ethelbert Ward Wolstenholme OBE FRCP was a British medical doctor, and the founding director of the Ciba Foundation. He was born in Sheffield, the son of a mechanical engineer, and educated at Repton School and Corpus Christi, Cambridge.

CIBA FOUNDATION COLLOQUIA ON ENDOCRINOLOGY 5
CONTENTS 9
Foreword by the Chairman 17
Requirements for clinically useful endocrine bioassays 19
Discussion 25
The bioassay of thyrotrophic hormone in the, hypophy- sectomized rat using 32P 28
Assay of thyrotrophic hormone by 131I discharge 38
Chick assay of thyrotrophic hormone using 32P 47
Discussion 51
Effects of chorionic and equine gonadotrophins on hypophy- sectomized immature rats 55
Discussion 66
Chromatography of urinary gonadotrophins 68
Discussion 73
Problems in the clinical use of gonadotrophins 76
Discussion 81
Clinical applications of the assay of pituitary and placental gonadotrophins 82
Discussion 94
Tests for luteotrophin 98
Discussion 110
Some observations on prolactin assays by the pigeon crop-weight method 114
Discussion 127
Assay of urinary prolactin activity 130
Discussion 137
Bioassay of growth hormone 141
Discussion 148
Assay of growth hormone for diabetogenic activity 150
Discussion 156
The action of pituitary preparations on the adrenal cortex 159
Discussion 169
Attempts to assay ACTH without hypophysectomy 173
Discussion 176
The measurement of ACTH activity in plasma 179
Discussion 184
Eosinophils, lymphocytes, and 17-hydroxycorticosteroids as indices of adrenocortical activity 188
Discussion 196
The effect of adrenal steroids upon muscle work 201
Discussion 210
Observations on comparative biological assays of adrenal cortical steroids 212
Discussion 217
Assay of gluco-corticoids 219
Discussion 226
The paper chromatography of steroids and its application to assay problems 229
Discussion 239
Determination of 3-ketosteroids as a measure of adrenal function 242
Discussion 246
Chairman's Closing Remarks 248