Amyloid, Prions, and Other Protein Aggregates, Part B (eBook)

Cover 1
Title Page 4
Copyright Page 5
Table of Contents 6
Contributors to Volume 412 10
Preface 14
Volumes in Series 18
Section I: Characterization of Protein Deposition In Vivo and Ex Vivo 42
Chapter 1. Protein Misfolding Cyclic Amplification for Diagnosis and Prion Propagation Studies 44
Chapter 2. Fractionation of Prion Protein Aggregates by Asymmetrical Flow Field-Flow Fractionation 62
Chapter 3. Analysis of Amyloid Aggregates Using Agarose Gel Electrophoresis 74
Chapter 4. Characterization of Systemic Amyloid Deposits by Mass Spectrometry 89
Chapter 5. Proteomics of Polyglutamine Aggregates 104
Chapter 6. Merger of Laser Capture Microdissection Mass Spectrometry: A Window into the Amyloid Plaque Proteome 118
Chapter 7. MALDI MS Imaging of Amyloid 135
Chapter 8. Imaging Polyglutamine Deposits in Brain Tissue 147
Chapter 9. X-34 Labeling of Abnormal Protein Aggregates During the Progression of Alzheimer’s Disease 164
Chapter 10. Visualizing Pathology Deposits in the Living Brain of Patients with Alzheimer’s Disease 185
Chapter 11. Micro-Imaging of Amyloid in Mice 202
Section II: Cell and Animal Models of Amyloid Formation and Toxicity 224
Chapter 12. An Ef.cient Protein Transformation Protocol for Introducing Prions into Yeast 226
Chapter 13. Screening for Genetic Modifiers of Amyloid Toxicity in Yeast 242
Chapter 14. Searching for Anti-Prion Compounds: Cell-Based High-Throughput In Vitro Assays and Animal Testing Strategies 264
Chapter 15. A Drosophila Model of Alzheimer’s Disease 275
Chapter 16. Modeling Polyglutamine Pathogenesis in C. elegans 297
Section III: Computational Approaches and Theory 324
Chapter 17. Nucleation: The Connections Between Equilibrium and Kinetic Behavior 326
Chapter 18. Amyloid Fibril Structure Modeling Using Protein Threading and Molecular Dynamics Simulations 341
Chapter 19. Ab initio Discrete Molecular Dynamics Approach to Protein Folding and Aggregation 355
Chapter 20. Computational Approaches to Fibril Structure and Formation 379
Author Index 408
Subject Index 436