Peptidases and Neuropeptide Processing (eBook)

Cover 1
Table of Contents 6
Contributors to Volume 23 10
Preface 14
Volumes in Series 16
Section I: Molecular Approaches for the Study of Intracellular Processing Enzymes 18
Chapter 1. Molecular Strategies for Identifying Processing Enzymes 20
Chapter 2. In Situ Hybridization Techniques to Map Processing Enzymes 33
Chapter 3. Analysis of Ontogeny of Processing Enzyme Gene Expression and Regulation 62
Chapter 4. Use of Vaccinia Virus Vectors to Study Neuropeptide Processing 82
Chapter 5. Overexpression of Neuropeptide Precursors and Processing Enzymes 111
Chapter 6. Use of Antisense RNA to Block Peptide-Processing Enzyme Expression 126
Section II: Immunological and Biochemical Approaches to the Study of Peptide-Processing Pathways 140
Chapter 7. Combination of High-Performance Liquid Chromatography and Radioimmunoassay for Characterization of Peptide-Processing Pathways 142
Chapter 8. Development and Use of Two-Site Immunometric Assays for Examining Peptide-Processing Pathways 157
Chapter 9. Methods for Identification of Neuropeptide-Processing Pathways 172
Chapter 10. Immunological and Related Techniques for Studying Neurohypophyseal Peptide-Processing Pathways 212
Chapter 11. Approaches to Assessing Ontogeny of Processing Enzymes 225
Chapter 12. Measurement, Distribution, and Subcellular Localization of Peptide-Amidating Activity 236
Chapter 13. Methods for Studying Carboxypeptidase E 254
Chapter 14. Characterization of Endothelin-Converting Enzymes 268
Chapter 15. In Vivo Approaches for Studying Peptide Processing 283
Section III: Identification and Characterization of Extracellular Processing Enzymes in the Central Nervous System 296
Chapter 16. Identification and Characterization of Central Nervous Peptidase Activities 298
Chapter 17. Strategies for Characterizing, Cloning, and Expressing Soluble Endopeptidases 313
Chapter 18. Proteolytic Processing and Amyloid Protein Precursor of Alzheimer's Disease 334
Chapter 19. Strategies for Measurement of Angiotensin and Bradykinin Peptides and Their Metabolites in Central Nervous System and Other Tissues 345
Chapter 20. Distribution and Roles of Endopeptidase 24.11 361
Chapter 21. Identification and Distribution of Endopeptidase 24.16 in the Central Nervous System 380
Chapter 22. Autoradiographic Techniques to Map Angiotensin-Converting Enzyme in Brain and Other Tissues 400
Index 416
Color Plate Section 426