Advances in Clinical Chemistry (eBook)

Front Cover 1
Advances in Clinical Chemistry, Volume 33 4
Copyright Page 5
Contents 6
Contributors 8
Preface 10
Chapter 1. Red Blood Cell Enzymes and Their Clinical Application 12
1. Introduction 13
2. Structure and Function of Major Red Blood Cell Enzymes 17
3. Hereditary Hemolytic Anemia Associated with Red Blood Cell Enzyme Deficiency 25
4. Hereditary Nonhemolytic Disorders Associated with Red Blood Cell Enzyme Deficiency 42
5. Hereditary Nonhematologic Disorders That Can Be Diagnosed by the Determination of Red Blood Cell Enzyme Activity 44
6. Summary 48
References 48
Chapter 2. Endogenous Mediators in Sepsis and Septic Shock 66
1. Introduction 66
2. Sepsis and Cytokines: Current Status 69
3. Vasoactive Agents 80
4. Hemostasis in Sepsis 86
5. Hormonal Regulation 97
6. The Interplay of Mediators in Sepsis 114
References 118
Chapter 3. Current Concepts of Coagulation and Fibrinolysis 144
1. Introduction 145
2. Basic Concepts of Coagulation 145
3. Basic Concepts of Fibrinolysis 153
4. Anticoagulant Therapy 158
5. Clinical Aspects 162
6. Nonanalytical Variables Affecting the Laboratory Evaluation of Hemostasis 168
7. Strategy for the Systematic Examination of Thrombophilia 173
8. Conclusion 174
References 174
Chapter 4. Tumor Markers: Reclassification and New Approaches to Evaluation 180
1. Introduction 180
2. Background 181
3. Reclassification 183
4. The Premarket Evaluation Process 186
5. Substantial Equivalence 188
6. A New 510(k) Paradigm 188
7. Comparison Studies 193
8. Tumor Marker 510(k) Principles and Applications 195
9. Tumor Marker Monitoring 196
10. The Role of Receiver Operating Characteristic (ROC) Curves in the Evaluation of Tumor Markers 197
11. Clinical Aspects and Applications of Tumor Markers 198
12. Conclusion 208
References 208
Chapter 5. Branched DNA Signal Amplification for Direct Quantitation of Nucleic Acid Sequences in Clinical Specimens 212
1. Introduction 212
2. Branched DNA 213
3. Signal versus Target Amplification Systems 223
4. Infectious Disease Applications 227
5. Quantitation of Messenger RNA 240
6. Summary 242
References 243
Index 248