Advances in Clinical Chemistry (eBook)

Front Cover 1
Advances in Clinical Chemistry, Volume 32 4
Copyright Page 5
Contents 6
Contrinutors 8
Preface 10
Chapter 1. Concepts Principles and Applications of Selected Molecular Biology Techniques in Clinical Biochemistry 12
1. Introduction 13
2. Basic Concepts 13
3. Principles of Selected Molecular Biology Techniques 15
4. Applications 38
5. Summary 44
References 45
Chapter 2. Clinical Molecular Biology: Concepts and Applications 50
1. Introduction 50
2. Molecular Technology and the Diagnosis of Diseases 54
3. Cystic Fibrosis 56
4. Duchenne and Becker Muscular Dystrophy 57
5. Lymphoproliferative Disorders 58
6. Chronic Myelogenous Leukemia and Acute Leukemias 59
7. Human Papilloma Virus 61
8. Clinical Applications of Molecular Technologies 61
9. Cystic Fibrosis: Current Diagnostics 65
10. Duchenne and Becker Muscular Dystrophy: Current Diagnostics 66
11. Lymphoproliferative Disorders: Current Diagnostics 67
12. Chronic Myelogenous Leukemia and Acute Leukemias: Current Diagnostics 68
13. Human Papilloma Virus: Current Diagnostics 69
14. Perspective on the Evaluation of Molecular Diagnostic Tests 69
15. Software Applications to Molecular Diagnostics 79
16. Quality Assurance: Scientific and Regulatory Issues 80
17. Conclusion 81
References 81
Chapter 3. Lipoprotein (a) 84
1. Introduction 85
2. General 85
3. Genetics 93
4. Metabolism 99
5. Lipoprotein (a) and Clinical Manifestations of Atherosclerosis 103
6. Lipoprotein (a), Thrombogenesis, and Fibrinolysis 106
7. Lipoprotein (a) and Other Pathological Conditions 112
8. Analysis of Lipoprotein (a) 116
9. Conclusion 121
References 122
Chapter 4. The Biochemistry of Metastasis 146
1. Introduction 146
2. Main Steps in Metastasis 147
3. Routes for the Dissemination of Cancer Cells 148
4. Organ Specificity of Metastases 149
5. Molecules Involved in Metastasis 150
6. Markers of Metastatic Potential 165
7. Conclusion 169
References 170
Chapter 5. AIDS: Biochemical Prospectives 150
1. Introduction 179
2. The Immune System 186
3. Virology and Human Immune Deficiency Virus 202
4. Infection with HIV 211
5. Laboratory Techniques for the Diagnosis of HIV Infection 229
6. Antiviral Inhibitory Therapy for HIV 238
7. Future Directions in Combating AIDS 243
References 249
Index 252