Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas (eBook)
492 Seiten
Elsevier Science (Verlag)
978-0-08-049520-0 (ISBN)
This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today.
This book is the second volume of three planned, individually-sold volumes on this topic. Like Volume 1, this book fully explains the principles and applications of modern techniques used in the field of molecular genetics. It will be of particular interest to pathologists and molecular pathologists conducting both academic and/or clinical research.
* The only book available that translates molecular genetics into cancer diagnosis
* The results of each Immunohistochemical and in situ hybridization method are presented in the form of color illustrations
* Methods discussed were either developed or refined by expert contributors in their own laboratories
Immunohistochemistry is the use of specific antibodies to stain particular molecular species in situ. This technique has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system, and has the potential to improve diagnosis, prognosis and therapeutic options of cancer. Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today. This book is the second volume of three planned, individually-sold volumes on this topic. Like Volume 1, this book fully explains the principles and applications of modern techniques used in the field of molecular genetics. It will be of particular interest to pathologists and molecular pathologists conducting both academic and/or clinical research. The only book available that translates molecular genetics into cancer diagnosis The results of each Immunohistochemical and in situ hybridization method are presented in the form of color illustrations Methods discussed were either developed or refined by expert contributors in their own laboratories
Cover 1
Contents 8
Authors and Coauthors of Volume 12
Foreword 16
Preface to Volume 2 18
Contents of Volume 1 20
Prologue 24
Selected Definitions 26
Part I: Molecular Pathology 34
Chapter 1.1. Laser Capture Microdissection- Microarray Technology. Global mRNA Amplification for Expression Profiling on Laser Capture Cells 36
Chapter 1.2. Comparative Genomic Hybridization Analysis Using Metaphase or Microarray Slides 44
Chapter 1.3. Microarray Immunoassay of Complex Specimens: Problems and Technologic Challenges 56
Chapter 1.4. Comparative Genomic Hybridization 70
Chapter 1.5. Microsatellite Instability in Cancer: Assessment by High Resolution Fluorescent Microsatellite Analysis 88
Chapter 1.6. The Role of Extreme Phenotype Selection in Cancer Research 98
Chapter 1.7. Rolling Circle Amplification 106
Chapter 1.8. Direct, in situ Assessment of Telomere Length Variation in Human Cancers and Preneoplastic Lesions 116
Chapter 1.9. Clinical Flow Cytometry of Solid Tumors 122
Chapter 1.10. Suppression Subtractive Hybridization Technology 146
Part II. Colorectal Carcinoma 160
Chapter 2.1. Colorectal Carcinoma: An Introduction 162
Chapter 2.2. Role of Immunohistochemical Expression of p53 in Colorectal Carcinoma 172
Chapter 2.3. Applying Tissue Microarray in Rectal Cancer: Immunostaining of Ki-67 and p53 182
Chapter 2.4. Role of Immunohistochemical Expression of p21 in Rectal Carcinoma 192
Chapter 2.5. Role of p107 Expression in Colorectal Carcinoma 196
Chapter 2.6. Expression of Gastric MUC5AC Mucin During Colon Carcinogenesis 200
Chapter 2.7. Role of Cyclooxygenase2 Expression in Colorectal Cancer 216
Chapter 2.8. Role of Immunohistochemical Expression of Bcl-2 in Colorectal Carcinoma 226
Chapter 2.9. Immunohistochemical Detection of CD97 Protein in Colorectal Carcinoma 234
Chapter 2.10. Roles of Immunohistochemical Expression of Cyclin A and Cyclin-Dependent Kinase 2 in Colorectal Tumors 240
Chapter 2.11. Role of Mismatch Repair Proteins and Microsatellite Instability in Colon Carcinoma 248
Chapter 2.12. Role of CD-61 (Beta-3 Integrin) Glycoprotein in Colon Carcinoma 260
Chapter 2.13. Immunohistochemical and in situ Hybridization Analysis of Lumican in Colorectal Carcinoma 270
Chapter 2.14. Role of Immunohistochemical Expression and in situ Hybridization Expression of Endothelin in Colon Carcinoma 278
Chapter 2.15. Role of Fibroblastic Stroma in Colon Carcinoma 288
Chapter 2.16. Role of Immunohistochemical Expression of p53, Rb, and p16 Proteins in Anal Squamous Cell Carcinoma 300
Part III: Prostate Carcinoma 310
Chapter 3.1. Prostate Carcinoma: An Introduction 312
Chapter 3.2. Genetic Alterations in Prostate Cancer 332
Chapter 3.3. Alterations of Genes and Their Expression in Prostate Carcinoma 340
Chapter 3.4. In situ Hybridization of Human Telomerase Reverse Transcriptase mRNA in Prostate Carcinoma 354
Chapter 3.5. Detection of Genetic Abnormalities Using Comparative Genomic Hybridization in Prostate Cancer Cell Lines 360
Chapter 3.6. Markers for the Development of Early Prostate Cancer 368
Chapter 3.7. The Role of MUC18 in Prostate Carcinoma 380
Chapter 3.8. Role of Immunohistochemical Expression of PCNA and p53 in Prostate Carcinoma 392
Chapter 3.9. Role of the p14ARFand p16INK4aGenes in Prostate Cancer 402
Chapter 3.10. P504S/a-Methylacyl CoA Racemase. A New Cancer Marker for the Detection of Prostate Carcinoma 410
Chapter 3.11. Role of Somatostatin Receptors in Prostate Carcinoma 420
Chapter 3.12. Role of Immunohistochemical Expression of Retinoid X Receptors in Prostate Carcinoma 432
Chapter 3.13. Role of Androgen Receptor Cofactors in Prostate Cancer 442
Chapter 3.14. Role of Androgen Receptor Gene Amplification and Protein Expression in Hormone Refractory Prostate Carcinoma 456
Chapter 3.15. Role of Immunohistochemical Loss of Bin1/Amphiphysin2 in Prostatic Carcinoma 464
Chapter 3.16. Role of Prostate-Specific Glandular Kallikrein 2 in Prostate Carcinoma 472
Chapter 3.17. Expression and Gene Copy Number Alterations of HER-2/neu (ERBB2) Gene in Prostate Cancer 482
Chapter 3.18. Combined Detection of Low Level HER-2/neu Expression and Gene Amplification in Prostate Cancer by Immunofluorescence and Fluorescence in situ Hybridization 490
Chapter 3.19. Calpain Proteases in Prostate Carcinoma 496
Chapter 3.20. Immunohistochemical Expression of Raf Kinase Inhibitor Protein in Prostate Carcinoma 504
Index 514
| Erscheint lt. Verlag | 31.1.2005 |
|---|---|
| Sprache | englisch |
| Themenwelt | Sachbuch/Ratgeber |
| Medizin / Pharmazie ► Allgemeines / Lexika | |
| Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie | |
| Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| ISBN-10 | 0-08-049520-6 / 0080495206 |
| ISBN-13 | 978-0-08-049520-0 / 9780080495200 |
| Haben Sie eine Frage zum Produkt? |
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