Annual Reports on NMR Spectroscopy (eBook)

Front Cover 1
Annual Reports on NMR Spectroscopy 4
Copyright Page 5
Contents 6
Contributors 10
Preface 12
Chapter 1: NMR of Antimicrobial Peptides 14
1. Introduction 15
2. Mechanism of Action 17
3. Solution-State NMR 21
4. Solution-State NMR Experiments 23
5. Solvent Considerations 26
6. Structures of Antimicrobial Peptides in Micelles 29
7. Organic Membrane Mimetic Solvents 32
8. Orientation in Detergent Micelles 36
9. Structure Determination of Larger Complexes 39
10. Endotoxin Binding by Antimicrobial Peptides 42
11. Recombinant Expression of Antimicrobial Peptides 42
12. Solid-State NMR of Antimicrobial Peptides 45
13. Peptide Orientation in Bilayers 46
14. Depth of Membrane Insertion 50
15. Oligomerization of Antimicrobial Peptides 51
16. Bilayer Perturbations 52
17. Conclusions 57
Acknowledgments 58
References 58
Chapter 2: NMR Studies of Protein Folding: Folding Studies of Calcium-Binding Lysozyme and alpha-Lactalbumin 66
1. Introduction 67
2. Hydrogen-Deuterium Exchange 69
3. Structure and Dynamics of Unfolded States of Proteins 81
4. Hydrogen Exchange Pulse Labelling and Real-Time NMR 85
5. Concluding Remarks 87
References 87
Chapter 3: Applications of the NOE in Molecular Biology 90
1. Introduction 91
2. The Basics 91
3. Faster and Better 102
4. Complexes 110
5. Interactions with Water 114
6. NOEs Involving Nuclei Other than 1H 117
Abbreviations 118
Acknowledgment 118
References 118
Chapter 4: Recent Applications of REDOR to Biological Problems 124
1. Introduction 125
2. Applications with Peptides 125
3. Applications with Peptides in Membranes 132
4. Other Applications 139
5. Conclusions and Summary of Earlier Applications 147
Acknowledgments 148
References 148
Chapter 5: Electrophoretic NMR-Ions, Molecules, Mixtures, Pores and Complexes 152
1. Theoretical and Historical Perspectives 153
2. Practical Aspects 153
3. Investigations into Small and Large Molecule Mobilities, and Complex Formation 157
4. Determination of Small Molecule and Ion Mobility 167
5. Outlook 170
References 171
Chapter 6: Two-Dimensional Magnetic Resonance Spectroscopy on Biopsy and In Vivo 174
1. Introduction 175
2. Assignments 176
3. Spectral Filtering 180
4. Analysis of Human Biopsies by COSY 182
5. Colon 188
6. Pre-Invasive Ovarian Lesions Identified by COSY 189
7. In Vivo 2D MRS 192
8. Human Muscle 195
9. Human Bone Marrow 197
10. Prostate 197
11. Brain 201
12. Breast 201
13. 2D Spectral Fitting (ProFit) 207
14. Conclusions 207
Abbreviations 208
References 210
Subject Index 214