Flow and Image Cytometry

Image analysis and flow cytometry are complementary techniques which provide new insights into essential aspects of cell biology. This book provides an up-to-date overview of current ideas and methods in these areas. The first part of the book deals with adhesion molecules, membrane pump dynamics, membrane fusion and endocytosis, membrane potential, T-cell homing and cytoskeleton. The second part focuses on problems of cell proliferation, chromosome analysis and sorting, apoptosis, imaging cytometry of fixed and living cells, image analysis for gene mapping from flow-sorted chromosomes, chromatin organization, and liposome-mediated delivery of DNA binding drugs. The final part covers data management systems, cell sorting techniques and microscopy.