SUMO Protocols

The SUMO System: An Overview.- Identification of Sumo Targets.- Identification of SUMO Target Proteins by Quantitative Proteomics.- Identification of SUMO-Conjugated Proteins and their SUMO Attachment Sites Using Proteomic Mass Spectrometry.- Identification of SUMO Targets Through In Vitro Expression Cloning.- Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS).- In Vivo Detection and Characterization of Sumoylation Targets in Saccharomyces cerevisiae.- Identification Of Sumo Interactors.- Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis.- Identification of SUMO-Binding Motifs by NMR.- Functional Analysis Of Sumoylation In Vivo.- Regulation of Transcription Factor Activity by SUMO Modification.- Characterization of the Effects and Functions of Sumoylation Through Rapamycin-Mediated Heterodimerization.- Biochemical Studies Of Sumo Conjugation.- Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation.- Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes.- Preparation of Sumoylated Substrates for Biochemical Analysis.- Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli.- Biochemical Studies of Sumo Deconjugation.- Preparation of SUMO Proteases and Kinetic Analysis Using Endogenous Substrates.- An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage.- FRET-Based In Vitro Assays for the Analysis of SUMO Protease Activities.- Detection and Characterization of SUMO Protease Activity Using a Sensitive Enzyme-Based Reporter Assay.- Manipulation And Alternative Uses Of The Sumo System.- Inhibition of the SUMO Pathway by Gam1.- SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems.