Protein Secretion

The Extraordinary Diversity of Bacterial Protein Secretion Mechanisms.- In Vitro and In Vivo Approaches to Studying the Bacterial Signal Peptide Processing.- Membrane Insertion of Small Proteins.- Membrane Protein Insertion in E. coli.- Study of Polytopic Membrane Protein Topological Organization as a Function of Membrane Lipid Composition.- In Vivo Analysis of Protein Translocation to the Escherichia coli Periplasm.- Sorting of Bacterial Lipoproteins to the Outer Membrane by the Lol System.- Purification and Functional Reconstitution of the Bacterial Protein Translocation Pore, the SecYEG Complex.- Reconstitution of the SecY Translocon in Nanodiscs.- In Vitro Assays to Analyze Translocation of the Model Secretory Preprotein Alkaline Phosphatase.- Characterization of Interactions Between Proteins Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance Spectroscopy.- Analysis of Tat Targeting Function and Twin-Arginine Signal Peptide Activity in Escherichia coli.- Site-Specific Cross-Linking of In Vitro Synthesized E. coli Preproteins for Investigating Transmembrane Translocation Pathways.- Tracking the Secretion of Fluorescently Labeled Type III Effectors from Single Bacteria in Real Time.- Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-PAGE.- Using Hidden Markov Models to Discover New Protein Transport Machines.- Bioinformatics Predictions of Localization and Targeting.- The Chloroplast Protein Import Machinery: A Review.- Measurement of the Energetics of Protein Transport Across the Chloroplast Thylakoid Membrane.- In Vitro Dissection of Protein Translocation into the Mammalian Endoplasmic Reticulum.- In Vitro Reconstitution of the Selection, Ubiquitination, and Membrane Extraction of a Polytopic ERAD Substrate.- Studying the ArfGAP-Dependent Conformational Changes in SNAREs.- Studying Endoplasmic Reticulum Function In Vitro Using siRNA.- High-Quality Immunofluorescence of Cultured Cells.- TrappingOxidative Folding Intermediates During Translocation to the Intermembrane Space of Mitochondria: In Vivo and In Vitro Studies.- Native Techniques for Analysis of Mitochondrial Protein Import.