HIV Protocols

Preparation of Virus Particles and their Analysis.- Methods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 Genomes.- Purification of HIV-1 Virions by Subtilisin Digestion or CD45 Immunoaffinity Depletion for Biochemical Studies.- Calculating HIV-1 Infectious Titre Using a Virtual TCID50 Method.- Methods To Study Hiv-1 Replication.- Cell-Free Assays for HIV-1 Uncoating.- Real-Time PCR Analysis of HIV-1 Replication Post-entry Events.- Analysis of 2-LTR Circle Junctions of Viral DNA in Infected Cells.- HIV-1 Recombination: An Experimental Assay and a Phylogenetic Approach.- Methods of Preparation and Analysis of Intracellular Reverse Transcription Complexes.- Analysis of Viral and Cellular Proteins in HIV-1 Reverse Transcription Complexes by Co-immunoprecipitation.- Isolation and Analysis of HIV-1 Preintegration Complexes.- Bisarsenical Labeling of HIV-1 for Real-Time Fluorescence Microscopy.- Methods for the Study of HIV-1 Assembly.- Assembly of Immature HIV-1 Capsids Using a Cell-Free System.- Preparation of Recombinant HIV-1 Gag Protein and Assembly of Virus-Like Particles In Vitro.- Methods for the Analysis of HIV-1 Nucleocapsid Protein Interactions with Oligonucleotides.- Methods for Analysis of Incorporation and Annealing Methods for Analysis of tRNALys in HIV-1.- Specialized Approaches To Study Hiv-1 Biology and Pathogenesis.- Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors.- Rapid, Controlled and Intensive Lentiviral Vector-Based RNAi.- Reverse Two-Hybrid Screening to Analyze Protein–Protein Interaction of HIV-1 Viral and Cellular Proteins.- Methods to Study Monocyte Migration Induced by HIV-Infected Cells.- Novel Mouse Models for Understanding HIV-1 Pathogenesis.- Immunological Studies Of Hiv.-Mucosal Antibody Responses to HIV.- Isolating Mucosal Lymphocytes from Biopsy Tissue for Cellular Immunology Assays.- Quantifying HIV-1-Specific CD8 + T-Cell Responses Using ELISPOT and Cytokine Flow Cytometry.- Multiparameter Flow Cytometry Monitoring of T Cell Responses.- Measuring HIV Neutralization in a Luciferase Reporter Gene Assay.- Assessing the Antiviral Activity of HIV-1-Specific Cytotoxic T Lymphocytes.- Methods for Quantitating Antigen-Specific T Cell Responses Using Functional Assays in Rhesus Macaques.- Drug Resistant Viruses And Viral Evolution.- Isolation of Drug-Resistant Mutant HIV Variants Using Tissue Culture Drug Selection.- Virus Evolution as a Tool to Study HIV-1 Biology.