Molecular Embryology

The Mouse Embryo.- The Mouse as a Developmental Model.- Culture of Postimplantation Mouse Embryos.- Organ Culture in the Analysis of Tissue Interactions.- Treatment of Mice with Retinoids In Vivo and In Vitro.- Analysis of Skeletal Ontogenesis through Differential Staining of Bone and Cartilage.- Cell Grafting and Labeling in Postimplantation Mouse Embryos.- Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs.- Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells.- Gene Trapping in Mouse Embryonic Stem Cells.- Application of lacZ Transgenic Mice to Cell Lineage Studies.- Transgenic RNA Interference to Investigate Gene Function in the Mouse.- Mouse Primordial Germ Cells.- Gene Transfer to the Rodent Embryo by Retroviral Vectors.- The Chicken Embryo.- The Avian Embryo.- Chick Embryos.- New Culture.- EC Culture.- Grafting Hensen's Node.- Grafting of Somites.- Microsurgical Manipulation of the Notochord.- Transplantation of Avian Neural Tissue.- Grafting of Apical Ridge and Polarizing Region.- Tissue Recombinations in Collagen Gels.- Quail—Chick Chimeras.- Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo.- Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses.- Electroporation in Avian Embryos.- Amphibian Embryos.- An Overview of Xenopus Development.- Mesoderm Induction Assays.- Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian.- A Method for Generating Transgenic Frog Embryos.- Axolotl/Newt.- Zebrafish And Medaka.- The Zebrafish.- Small-Scale Marker-Based Screening for Mutations in Zebrafish Development.- Microinjection and Cell Transplantation in Zebrafish Embryos.- Recent Advances inMeganuclease-and Transposon-Mediated Transgenesis of Medaka and Zebrafish.- Retinoids in Nonmammalian Embryos.- Nonvertebrate Chordates.- Protochordates.- Molecular Techniques.- Subtractive Hybridization and Construction of cDNA Libraries.- Differential Display of Eukaryotic mRNA.- Using DNA Microarrays.- Profiling Gene Transcription in the Developing Embryo.- RT-PCR on Embryos Using Degenerate Oligonucleotide Primers.- Single-Cell RT-PCR cDNA Subtraction.- In-Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections.- In-Situ Hybridization and Immunohistochemistry in Whole Embryos.- Wholemount In Situ Hybridization to Xenopus Embryos.- Wholemount In Situ Hybridization to Amphioxus Embryos.- In-Situ Hybridization to Sections (Nonradioactive).- Immunohistochemistry Using Polyester Wax.- Immunohistochemistry on Whole Embryos.- Whole Embryo Assays for Programmed Cell Death.- Protein Techniques.- Microscopy and Photography.- Microscopy and Photomicrography Techniques.