Enzyme Kinetics and Mechanism

Paul F. Cook is the Grace B. Kerr Centennial Professor of Chemistry and Biochemistry at the University of Oklahoma. His awards and achievements twice included the Alexander von Humboldt Research Fellowship and he was the recipient of the National Institutes of Health Research Career Development Award from 1983 to 1988. Professor Cook is Associate Editor of Protein and Peptide Letters and Executive Editor of Proteins and Proteomics. W.W. Cleland is the M.J. Johnson Professor and Steenbock Professor of Chemical Science at the Enzyme Institute at University of Wisconsin-Madison. In the early 1960s, Cleland's landmark papers created a theoretical framework for the study of steady-state kinetics of multisubstrate enzymes and laid the foundation for all subsequent steady-state studies. He serves on the editorial boards of Journal of Biological Chemistry and of Biochemistry. Recognized by many as the "father of modern enzyme kinetics," Professor Cleland was elected to the National Academy of Sciences in 1985.

1. Nomenclature
2. Introduction to Kinetics
3. Enzyme Assays
4. Derivation of Initial Velocity Rate Equations and Data Processing
5. Initial Velocity Studies in the Absence of Added Inhibitors
6. Initial Velocity Studies: Presence of Added Inhibitors
7. Pre-Steady State and Relaxation Kinetics
8. Isotopic Probes of Kinetic Mechanism
9. Isotope Effects as a Probe of Mechanism
10. pH Dependence of Kinetic Parameters and Isotope Effects
Appendix I. King and Altman Patterns and Distribution Equations
Appendix II. Rate Equations, Definitions of Kinetic and Inhibition Constants, Haldanes, Distribution Equations and Rate Constants for a Number of Multireactant Mechanisms