Hepatitis B and D Protocols

Part I. Detection of Viral Markers; Overview of Commercial HBV Assay Systems Stefan Zeuzem; Detection of HBV DNA in Serum Using a PCR-Based Assay Hau Tim Chung; Detection of HBV DNA by Oligonucleotide Probing Hsiang Ju Lin; Detection of HBV RNA in Serum of Patients Wei Zhang, Hans Jorg Hacker, Maria Mildenberger, Qin Su, and Claus H. Schroder; Quantification of HBV Covalently Closed Circular DNA from Liver Tissue by Real-Time PCR Scott Bowden, Kathy Jackson, Margaret Littlejohn, and Stephen Locarnini; In Situ Hybridization for the Detection and Localization of HBV DNA in Liver Sections Vicky C. H. Lai and Johnson Y. N. Lau; Quantitative Assay of Hepatitis B Surface Antigen in Serum or Plasma Using Laurell Electrophoresis Wolfram H. Gerlich, Ulrike Wend, and Dieter Glebe; In Situ Detection of Hepatitis B Viral Antigens: An Immunohistochemical Approach Jane W. S. Fang and Johnson Y. N. Lau; Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting Jie Liu and Mark A. Feitelson; Detection of Serum HDV-RNA by RT-PCR Antonina Smedile, Maria Grazia Niro, and Mario Rizzetto; Nonradioisotopic In Situ Hybridization for HDV RNA Francesco Negro; Detection and Characterization of Small and Large HDV Antigens Thomas B. Macnaughton and Michael M. C. Lai; Immunohistochemical Detection of Hepatitis Delta Antigen Karim Abid and Francesco Negro; Part II. Viral Genotypes and Variants; HBV Vaccine-Escape Variants Ashraf A. Basuni and William F. Carman; HBV Genotyping and Analysis for Unique Mutations Anna Ayres, Stephan Locarnini, and Angeline Bartholomeusz; A One-Filter-Three-Probe Assay for Defective Interference (DI) Effects of Naturally Occurring Core Internal Deletion (CID) Variants of Human Hepatitis B Virus Chiaho Shih and Ta-Tung Thomas Yuan; Detection of Hypermodified Middle-Envelope (M) Proteins Secreted from Naturally Occurring HBV Variants Containing a preS2 Internal Deletion Chiaho Shih and Pei-Ching Tai; Hepatitis B Viral Genotyping with the Research INNO-LiPA HBV Genotyping Line Probe Assay Sija De Gendt, Fred Shapiro, Jelena Juras, Els Van Assche, Geert Maertens, and Erwin Sablon; Part III. Molecular Biological Characterization; Processing of Hepatitis B Virus Surface Proteins Volker Bruss; Binding of Duck Carboxypeptidase D to Duck Hepatitis B Virus Stephan Urban; Nucleoprotein Transport of HBV Capsid Particles Michael Kann; Phosphorylation Analysis of Hepatitis B Virus Core Protein in Mammalian cells Jie Li, Yanyan Zheng, Jinah Choi, and Jing-hsiung Ou; Study of HBV Replication Capacity in Relation to Sequence Variation in the Precore and Core Promoter Regions Fabien Zoulim, ShuPing Tong, and Christian Trepo; A cis/trans Genetic Test for Pleiotropic Phenotypes Associated with a Frequent Naturally Occurring Mutation at Amino Acid 97 of HBV Core Protein Chiaho Shih and Ta-Tung Thomas Yuan; Studying DHBV Polymerase by In Vitro Transcription and Translation Jianming Hu; Expression and Purification of Functional Hepatitis B Virus Polymerase in the Baculovirus Insect Cell System Lisa Lott, Lena Notvall, and Robert E. Lanford; Localization of Duck Hepatitis B Virus Polymerase Within Cells Ermei Yao and John E. Tavis; Endogenous Polymerase Assay for the Analysis of Hepatitis B Virus in Transgenic Mice Zhenming Xu and Jing-hsiung Ou; Transcriptional Control of Hepatitis B Virus Anneke K. Raney; In Vitro Reconstitution of [epsilon]-Dependent Duck Hepatitis B Virus Replication Initiation