Target Identification and Validation in Drug Discovery

A Systemic View of Target Identification: Modeling the Warburg Effect.- Structural Biology for Target Identification and Validation.- Design and Preliminary Screen of Antisense Oligonucleotides.- Integration of RNAi and Small Molecule Library Screens to Identify Targets for Drug Discovery.- A Robust siRNA Screening Approach with Optimized Conditions for Large-Scale Transfection in Multiple Human Cancer Cell Lines.- Functional Lineage Tracing Using CaTCH.- Exploring the Perturbation of Biological Processes Caused by Gene Upregulation Using Knock-In Transfection, Transcriptomics, and Bioinformatics Analysis.- A Reverse Engineering Approach to Optimize Chemical Synergy Between Target and Phenotype: Bridging the Cancer and Malaria Indications.- High-Content Imaging-Based Screening for Anti-Cancer Compounds in Zebrafish Xenografts.- Identification of Protein Kinase Drug Targets Using Activity Estimation in Clinical Phosphoproteomics.- Click Chemistry for Target Engagement Studies.- The Power of Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS) in Structural Biology and Protein-Ligand Interactions.- Advancing Target Validation and Ligandability Assessment: Fragment Screening via 19F NMR Spectroscopy.- In Vivo Micro-CT Imaging for Quantitative Longitudinal Assessment of Pulmonary Diseases in Small Animals.- The NeuroSphere Assay Applied to Neural Stem Cells and Cancer Stem Cells.- High Efficiency Lentiviral Transduction of Colon Organoids Using Reversible 2D/3D Culture Techniques.- 3D Tumor Model to Study Immune Cell Infiltration.