Immuno-oncology and immunotherapy Part C

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

1. Extraction and quantification of histones from human cells
Luis de la Cruz-Merino, Victor Sanchez-Margalet Sr. and LOURDES HONTECILLAS PRIETO
2. Expression and characterization of Phosphatidylserine-targeting antibodies for biochemical and therapeutic applications
Raymond Birge and Varsha Gadiyar
3. ILC differentiation from HSCs in vitro
Lorenzo Moretta, Quatrini Linda, Francesca Romana Mariotti, Cecilia Ciancaglini and Silvia Santopolo
4. Methods to expande human Treg cells and assay their function
Juan José Lasarte and Teresa Lozano
5. Monitoring rapid activation of human gamma/delta T cells by multicolor flow cytometry
Dieter Kabelitz, Christin Peters, Jara Simeonov and Daniel Gombert
6. Methods to induce T cell exhaustion in vitro
Santos Manes, Rosa Ana Lacalle, Raquel Blanco and Rebeca García-Lucena
7. Ex vivo assessment of human neutrophil motility and migration
Karin E. de Visser, Claudia Burrello and Noor A.M. Bakker
8. Flow cytometry-based monitoring of myeloid-derived suppressor cells in the peripheral blood of patients with solid tumors
Udo S. Gaipl, Anna-Jasmina Donaubauer, Ilka Scheer, Rainer Fietkau and Benjamin Frey
9. Deciphering human blood and tumor neutrophil heterogeneity: Methods for isolation and assessing suppression of T-cell proliferation
Pierre van der Bruggen, Thibault Hirsch, Nicolas Delhez, Frank Aboubakar Nana, Camille Houbion, Alexandre Bayard, Annika Bruger, Christophe Vanhaver and Sven Brandau
10. Splenocyte anticancer citotoxicity assessment after prophylactic vaccination or drug treatment of tumor-bearing mice
Ana Carolina Martinez Torres, Kenny Misael Calvillo-Rodriguez, Ana Luisa Rivera-Lazarin, Reyes Tamez-Guerra and Cristina Rodriguez-Padilla
11. Therapeutic treatment of tumor-bearing mice with drug-killed cancer cells: a method to confirm immunogenic cell death and assess its therapeutic effectiveness
Ana Carolina Martinez Torres, Reyes Tamez-Guerra, Cristina Rodriguez-Padilla, Kenny Misael Calvillo-Rodriguez, Maria Norma Gonzalez-Flores and Marilena Antunes-Ricardo
12. RNA flow for intratumoral cytokines/chemokines
Romina Goldszmid and Khiem Lam
13. Multidimensional profiling of cancer microenvironments via imaging mass cytometry
Noel de Miranda
14. Evaluation of lymphocyte infiltration into tumor spheroids using immunofluorescence and confocal microscopy
Andreas Lunqdvist
15. In vivo imaging of tumor hypoxia and blood vessels integrity in small animals
Bassam Janji
16. Retrovirus-based manufacturing of CAR-modified T cells for cancer therapy research
Sebastian Kobold