Bone Research Protocols

Isolation and Culture of Osteoblasts.- Isolation and Generation of Osteoclasts.- Isolation, Purification, Generation, and Culture of Osteocytes.- Isolation, Differentiation, and Characterization of Human Bone Marrow Stromal Stem Cells In Vitro and In Vivo.- Isolation and Characterization of Primary Bone Marrow Adipocytes in Rodent Models.- Isolation and Characterization of Circulating Tumor Cells.- 2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies.- Isolation of Extracellular Vesicle Subtypes from Primary Osteoblast Cultures.- Using Cell and Organ Culture Models to Analyze Responses of Bone Cells to Mechanical Stimulation.- Analysis of Signaling Pathways by Western Blotting and Immunoprecipitation.- Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization.- Analysis of Transcriptional Regulation in Bone Cells.- The Ex Vivo Organ Culture of Bone and Cartilage.- Ex Vivo Model Systems of Cancer-Bone Cell Interactions.- Mechanical Properties of Bone Ex Vivo.- Ovariectomy/Orchidectomy in Rodents.- Osteoarthritis Mouse Model of Destabilization of the Medial Meniscus.- Models of Rheumatoid Arthritis.- In Vivo Models of Mechanical Loading.- Pre-Clinical Murine Models of Bone Sarcomas.- Mouse Models of Breast Cancer Bone Metastasis.- Radiographic Assessment of Osteosclerotic Lesions in Mice with Bone Metastasis.- Histomorphometry in Rodents.- Immunostaining of Skeletal Tissues.- Live Cell Imaging of Bone Cell and Organ Cultures.- Analysis of Bone Architecture in Rodents Using Micro Computed Tomography.- 3D Morphometry of Preclinical Bone Disease Models Beyond Osteoporosis.- MicroCT Scanning and Analysis in the Zebrafish Skeleton.- Ex Vivo Examining Single Cancer Cells Homing to Murine Bone Using Two-Photon Microscopy.- Quantitative X-Ray Imaging of Mouse Bone by Faxitron.- Scanning Electron Microscopy and Bone.- Fourier Transform Infrared Imaging of Bone.- Raman Microscopy and Bone.- Analysis of Genome-Wide Association Studies.- Analysis of Epigenome-Wide Association Studies.- Application of Systematic Review and Meta-Analysis in Musculoskeletal Research.