Nutrient Sensing in Eukaryotes

Measuring Cellular Adenine Nucleotides by Liquid Chromatography Coupled Mass Spectrometry.- FRET-Based Sensor for Measuring Adenine Nucleotide Binding to AMPK.- Monitoring Cellular Energy Balance in Single Cells Using Fluorescent Biosensors for AMPK.- Assays of Different Mechanisms of AMPK Activation, and Use of Cells Expressing Mutant AMPK to Delineate Activation Mechanisms.- Methods to Determine Lysosomal AMPK Activation.- Sensing of Long Chain Fatty Acyl-CoA Esters by AMPK.- Visualization of Subcellular mTOR Complex 1 Activity with a FRET-Based Sensor (TORCAR).- Determination of Nutrient Ligand - Sensor Binding Affinity.- Amino Acid Sensing by Transceptors: Exploring Substrate-Induced Regulation of Amino Acid Transporters and Transporter Expression.- Purification and Analysis of eIF2α Phosphorylation by Stress-Activated Protein Kinase Gcn2 from S. cerevisiae.- An In Vitro System to Study Mammalian GCN2 Regulation.- Sensing of Cholesterol by Squalene Monooxygenase.- Large Scale Protein Production and Activity Assay Protocols for Human Glycogen Synthase-Glycogenin Complex.- Measurement of Neurotransmitters and Metabolites in Cell Culture and In Vitro Using Genetically Encoded PBP-Based Biosensors.- Calcium Imaging in Hypothalamic Cells.- Nutrient Sensing by Lingual G-Protein Coupled Taste Receptors.