Protein Chromatography

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A Synopsis of Proteins and Their Purification.- Mixed-mode chromatography and its role in monoclonal antibody purification.- Application of Continuous Counter-current Chromatography in Protein Purification.- Chromatographic Purification of Viral Vectors for Gene Therapy Applications.- Scale-up of Protein Purification -Downstream Processing Issues.- Approaches to avoid Proteolysis During Protein Expression and Purification.- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.- Magnetic nanoparticles for protein separation and purification.- Ion-Exchange Chromatography: Basic Principles and Application.- Hydroxyapatite Chromatography.- Poly-Histidine-Tagged Protein Purification using IMAC.- Lectin Affinity Chromatography.- Single-step non-chromatographic purification of recombinant mammalian proteins using a split intein ELP tag system.- Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems.- Immunoprecipitation: Variations, Considerations, and Applications.- Protein Quantitation and Analysis of Purity.- Protein Extraction and Purification by Differential Solubilisation.- Protein Stability: Enhancement and Measurement.- Storage and Lyophilisation of Pure Proteins.- Strategies for the Purification of Membrane Proteins.